MÜRÜVVET AKÇAY ÇELİK, Yavuz ÖZORAN, Ümit ÇOBANOĞLU

Kolon Poliplerinde Tümör Supresör Gen (P53) ve Proliferatif İndeksin (Ki 67) Değerlendirilmesi

Amaç: Bu çalışmanın amacı, kolorektal poliplerde Ki 67 aktif proliferasyon varlığı, kriptte bulunduğu zon alanları, p53 ekspresyonları ile gen mutasyonlarının incelenmesi ve polip tiplerinin morfolojileri ile kıyaslı değerlendirilmesidir. Yöntem: Çalışmaya 142 kolon polipi alınarak histopatolojik nitelikleri Ki 67, p53 salınımları kaynak verileri ile farklı kanser yolaklarının farklı poliplerde incelenmesi amaçlanmıştır. Olguların tümü, Dünya Sağlık Örgütü (DSÖ) 2010 sınıflamasına göre yeniden değerlendirildi. Ki 67 ve p53 immün işaretleyicileri ile boyandı. Bulgular: Çalışmamızda, hiperplastik polipde (HP) Ki 67 ile kriptlerin alt zonunda %74,5 oranında boyanma saptandı. Adenomatöz polipde (AP, Adenom) ise Ki 67 ile kriptin hem alt zonunda (%40,9 oranında), hem üst zonunda (%56,1 oranında) boyanma saptandı. Hemen hemen tüm olgularda p53 ile kriptlerin 1/2 alt zonunda boyanma oranı düşük gözlendi. APde P53 ile kriptlerin 1/2 üst zonunda boyanma oranı %50 saptandı, HP ve sesil serrated adenom/polipde (SSA/P) boyanma oranı düşük saptandı. Sonuç: Olgu serolojik nitelikleri ile birlikte polipin histopatolojisi ve Ki 67, p53 ve moleküler genetik değerlendirmelerine paralel yapılacak çalışmalara gerek duyulmaktadır.

Evaluation of Proliferative Index (Ki 67) and Suppressor Gene (P53) Expression in Colon Polyps

Objective: The aim of this study is to evaluate the presence of Ki 67 proliferation zone of active areas and p53 mutation in colorectal polyps and to compare the results between the polyp types. Methods: Our study included 142 colon polyps. Studies based on the presence of dysplastic areas and dysmorphism in the polyp types that were considered to be nonneoplastic showed that a different pathway may have a role in malignant transformation. The cases were reclassified according to WHO 2010 classification. The tissue sections were stained by immunohistochemistry for Ki 67 and p53 Results: In our study Ki 67 staining was seen 74.5% in the lower zone of the crypt with HP. Ki 67 staining was seen in both the lower zone (40.9%) and the upper zone (56.1%) of the crypt with AP. In almost all cases, low rate of the p53 staining was observed in the ½ lower zone of crypts. The rate of p53 staining in the upper ½ zone of the crypts was 50% in AP cases. The staining ratio was low in HP and SSA/P Conclusion: The evaluation of polyp histopathology and Ki 67, p53, and molecular genetic with serologic characteristics of patients are required in parallel reviews

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ISSN: 1309-3878
Yayın Aralığı: 3
Başlangıç: 2009
Yayıncı: Düzce Üniversitesi Tıp Fakültesi Aile Hekimliği AD adına Yrd.Doç.Dr.Cemil Işık Sönmez