Mine GEÇGELEN CESUR, Tuna ÖNAL, Mehmet DİNÇER BİLGİN, Fevziye Burcu ŞİRİN, Sevinç İNAN, Ergun cem KÖKEN, Afra ALKAN, Gökhan CESUR

Histological and Biochemical Investigation of the Effects of Low Intensity Pulsed Ultrasound on Orthodontic Tooth Movement

Objective: The aim of this study is to investigate the effects of low intensity pulsed ultrasound on orthodontic tooth movement in rats. Methods: For this study, 40 12-week-old adult male Wistar albino rats from the Animal Laboratory at Adnan Menderes University, Faculty of Medicine, were used. Rats were divided into four groups of ten. Group 1 was the untreated control group. In group 2, an orthodontic spring was used to move teeth. In groups 3 and 4, orthodontic treatment was combined with low intensity pulsed ultrasound at 16 J/cm2 or 48 J/cm2, respectively, for 14 days. Tooth movement was measured on day 14. Serum bone alkaline phosphatase (BALP) and C-telopeptide of type I collagen (CTX-I) levels were analyzed biochemically. The number of osteoclasts, osteoblasts and inflammatory cells, capillary density and new bone formation was determined histologically. Receptor activator of nuclear factor-kappa B ligand (RANKL), osteoprotegerin (OPG), vascular endothelial growth factor (VGEF) and transforming growth factor-β (TGF-β) were assessed using immunohistochemical staining. Results: BALP and CTX-I levels in group 4 were significantly higher than for group 1. Tooth movement and the number of osteoclasts, inflammatory cells, and capillary density in group 4 were significantly greater than for group 2. The intensity levels of RANKL and OPG in group 4 were significantly greater than for group 2. Conclusion: Ultrasound is noninvasive application and a promising therapy for accelerating bone remodeling during orthodontic tooth movement.

Keywords:

Orthodontic treatment, low intensity pulsed ultrasound, tooth movement,

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ISSN: 1309-3878
Yayın Aralığı: 3
Başlangıç: 2009
Yayıncı: Düzce Üniversitesi Tıp Fakültesi Aile Hekimliği AD adına Yrd.Doç.Dr.Cemil Işık Sönmez