Mehmet FİDAN, Süleyman Mesut PINAR, Mehmet Emre EREZ, Behçet İNAL, Hüseyin EROĞLU

Balveren (Şırnak) Arıcılarının Konaklama Alanlarındaki Propolis Örneklerinin Botanik Kökeni ve Mineral İçeriğinin Belirlenmesi

Arı ürünleri ile ilgili çalışmalar gün geçtikçe artmaktadır. Aslında arı ürünlerinin içeriği, bileşeni; ekolojik ve floristik birçok faktöre bağlı olarak değişkenlik göstermekte, besleyici ve tedavi edici özelliği ise içeriği ile doğrudan ilişkilidir. Balveren beldesi (Şırnak) arıcıları kovanlarını; coğrafik yapısı, floristik ve topografik özellikleri farklı lokasyonlara yerleştirmektedirler. Bu değişkenlik balın kalitesini etkilediği gibi propolis özelliklerini de değiştirmektedir. Kovan yapıştırıcısı olarak bilinen propolis ile ilgili çalışmalar son yıllarda önem kazanmaktadır. Diğer arı ürünlerinde olduğu gibi, propolis içeriği de bölgenin floristik özelliklere bağlıdır. Bu çalışmada Balveren arıcılarının konakladıkları bölgelerden propolis örnekleri toplanmış, botanik orijinleri, mum oranları, fenolik madde içerikleri ve mineral madde miktarları analiz edilmiştir. Yapılan mikroskobik analizlerde arıların kullandığı 14 farklı familyaya ait polen taneleri tespit edilmiştir. Fenolik madde ve mineral madde içeriklerinin ise tamamen propolis örneği alınan lokasyona bağlı olarak değişkenlik gösterdiği tespit edilmiştir. Bu çalışma ile bölgede bulunan kovanların propolis özellikleri ortaya konulmaya çalışılmış ve yapılan çalışmanın bölge propolislerinin teknolojik ve tedavi edici amaçlar ile kullanılmasına yardımcı olması hedeflenmiştir.

Anahtar Kelimeler:

Arı, Polen, Toplam Fenolik, Balmumu, ICP OES

Determination of Botanical Origin and Mineral Content of Propolis Samples from Balveren (Şırnak) Beekeepers Accommodation Areas

Researches on bee products have become popular in recent years. In fact, the content and component of bee products varies depending on many ecological and floristic factors and its nutritional and therapeutic properties are directly related to its content. Balveren (Şırnak province) beekeepers place their hives in locations with different geographical structure, floristic and topographic characteristics. This variability not only affects the quality of honey but also changes the properties of propolis. Studies on propolis, known as bee glue, have gained importance in recent years. As with other bee products, the propolis content also depends on the floristic characteristics of the region. In this study, propolis samples were collected from the regions where Balveren beekeepers stayed and their botanical origins, wax ratios, phenolic content, and mineral substance contents were analyzed. In the microscopic analysis, pollen grains belonging to 14 different families used by bees were determined. It was determined that the total phenolic and mineral contents of propolis vary completely depending on the location. With this study, the propolis properties of the hives in the region were tried to be revealed and it was aimed that this study would help the region's propolis to be used for technological and therapeutic purposes.

Keywords:

Bee, Pollen, Total Phenolic, Wax, ICP OES,

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Abdullah, N.A., Ja'afar, F., Yasin, H.M., Taha, H., Petalcorin, M.I., Mamit, M.H., ..., & Usman, A. (2019). Physicochemical analyses, antioxidant, antibacterial, and toxicity of propolis particles produced by stingless bee Heterotrigona itama found in Brunei Darussalam. Heliyon, 5(9), 1-8. https://doi.org/10.1016/j.heliyon.2019.e02476
Aliyazıcıoglu, R., Sahin, H., Erturk, O., Ulusoy, E., & Kolayli, S. (2013). Properties of phenolic composition and biological activity of propolis from Turkey. International Journal of Food Properties, 16(2), 277-287. https://doi.org/10.1080/10942912.2010.551312
Anjum, S.I., Ullah, A., Khan, K.A., Attaullah, M., Khan, H., Ali, H., ..., & Dash, C.K. (2019). Composition and functional properties of propolis (bee glue): a review. Saudi Journal of Biological Sciences, 26(7), 1695-1703. https://doi.org/10.1016/j.sjbs.2018.08.013
Bagatir, G., Kaya, M., Suer, I., Cefle, K., Palanduz, A., Palanduz, S., Becerir, H. B., Koçyigit, M., & Ozturk, S. (2022). The effect of Anzer honey on X-ray induced genotoxicity in human lymphocytes: An in vitro study. Microscopy Research and Technique, 1–10. https://doi.org/10.1002/jemt.24081
Bankova, V., Pappaova M., & Trusheva B. (2014). Propolis volatile compounds: Chemical diversity and biological activity: a review. Chemistry Central Journal, 8, 28. https://doi.org/10.1186/1752-153X-8-28
Bankova, V., Trusheva, B., & Popova, M. (2021). Propolis extraction methods: a review. Journal of Apicultural Research, 60(5), 734-743. https://doi.org/10.1080/00218839.2021.1901426
Bonvehí, J.S., & Gutiérrez, A.L. (2011). Antioxidant activity and total phenolics of propolis from the Basque Country (Northeastern Spain). Journal of the American oil chemists' society, 88(9), 1387-1395. https://doi.org/10.1007/s11746-011-1792-1
Bonvehí, J.S., & Gutiérrez, A.L. (2011). Antioxidant activity and total phenolics of propolis from the Basque Country (Northeastern Spain). Journal of the American oil chemists' society, 88(9), 1387-1395. https://doi.org/10.1007/s11746-011-1792-1
Bonvehí, J.S., & Coll, F.V. (1994). Phenolic composition of propolis from China and from South America. Zeitschrift für Naturforschung c, 49(11-12), 712-718. https://doi.org/10.1515/znc-1994-11-1204
Borrelli, F., Maffia, P., Pinto, L., Ianaro, A., Russo, A., Capasso, F., & Ialenti, A. (2002). Phytochemical compounds involved in the anti-inflammatory effect of propolis extract. Fitoterapia, 73, S53-S63. https://doi.org/10.1016/S0367-326X(02)00191-0
Crane, E. (1999). History of other products from bees In: Crane, B. (ed) The world history of beekeeping and honey hunting, Routledge; New York; 545-553.
Cunha, I.B.S., Sawaya, A.C.H.F., Caetano, F.M., Shimizu, M.T., Marcucci, M.C., Drezza, F.T., Povia, G.S., & Carvalho, P. (2004). Factors that influence the yield and composition of Brazilian propolis extracts. Journal of the Brazilian Chemical Society, 15(6), 964-970.
Davis, P.H. (1971). Distribution patterns in Anatolia with particular reference to endemism. In Davis, P.H., Harper, P.C & Hedge, I.C. (eds) Plant Life of South-West Asia. Botanical Society of Edinburgh, Edinburgh. 15-27.
Dias, L.G., Pereira, A.P., & Estevinho, L.M. (2012). Comparative study of different Portuguese samples of propolis: pollinic, sensorial, physicochemical, microbiological characterization and antibacterial activity. Food and Chemical Toxicology, 50(12), 4246-4253. https://doi.org/10.1016/j.fct.2012.08.056
Formicki, G., Greń, A., Stawarz, R., Zyśk, B., & Gał, A. (2013). Metal content in honey, propolis, wax, and bee pollen and implications for metal pollution monitoring. Polish Journal of Environmental Studies, 22(1), 99-106.
Guzmán-Gutiérrez, S.L., Nieto-Camacho, A., Castillo-Arellano, J.I., Huerta-Salazar, E., Hernández-Pasteur, G., Silva-Miranda, M., ..., & Reyes-Chilpa, R. (2018). Mexican propolis: a source of antioxidants and anti-inflammatory compounds, and isolation of a novel chalcone and ε-caprolactone derivative. Molecules, 23(2), 334. https://doi.org/10.3390/molecules23020334
Herrera, C.L., Alvear, M., Barrientos, L., Montenegro, G., & Salazar, L.A. (2010). The antifungal effect of six commercial extracts of Chilean propolis on Candida spp. Ciencia e investigación agraria, 37(1), 75-84. http://doi.org/10.4067/S0718-16202010000100007
Kabata-Pendias, A. (2011). Trace elements in soils and plants, 4th edn. Boca Rato, CRC Press, 548 pp.
Kasote, D.M. (2017). Propolis: a neglected product of value in the Indian beekeeping sector. Bee World, 94(3), 80-83. https://doi.org/10.1080/0005772X.2017.1345223
Keskin, M., & Kolaylı, S. (2018). Standardization of propolis, Is it possible. Uludag bee journal, 18(2), 101-110. https://doi.org/10.31467/uluaricilik.485080
Kurek-Górecka, A., Rzepecka-Stojko, A., Górecki, M., Stojko, J., Sosada, M., & Świerczek-Zięba, G. (2014). Structure and antioxidant activity of polyphenols derived from propolis. Molecules, 19(1), 78-101. https://doi.org/10.3390/molecules19010078
Li, F., Awale, S., Tezuka, Y., & Kadota, S. (2008). Cytotoxic constituents from Brazilian red propolis and their structure–activity relationship. Bioorganic & Medicinal Chemistry, 16(10), 5434-5440. https://doi.org/10.1016/j.bmc.2008.04.016
Maden Çalışkol, M. (2013). Azerbaycan yöresine ait propolis örneklerinin antioksidan özelliklerinin belirlenmesi. Yüksek Lisans Tezi (332135). Retrieved from https://tez.yok.gov.tr/UlusalTezMerkezi/tezSorguSonucYeni.jsp
Negri, G., Marcucci, M.C., Salatino, A., & Salatino, M.L.F. (2000). Comb and propolis waxes from Brazil (states of São Paulo and Paraná). Journal of the Brazilian Chemical Society, 11, 453-457.
Oršolić, N., & Bašić, I. (2003). Immunomodulation by water-soluble derivative of propolis: a factor of antitumor reactivity. Journal of Ethnopharmacology, 84(2-3), 265-273. https://doi.org/10.1016/S0378-8741(02)00329-X
Ota, C., Unterkircher, C., Fantinato, V., & Shimizu, M.T. (2001). Antifungal activity of propolis on different species of Candida. Mycoses, 44(9‐10), 375-378. https://doi.org/10.1046/j.1439-0507.2001.00671.x
Özaltan, Z., & Koçyiğit, M. (2022). Pollen morphology of some taxa in the family Lamiaceae (Labiatae) from Turkey. EMU Journal of Pharmaceutical Sciences, 5(1), 11-20.
Özhatay, N., Kocyigit, M., & Bona, M. (2012). Istanbul’s Honey Plants. Istanbul. Turkey.
Perveen, A., & Qaiser, M. (2003). Pollen Morphology of the Family Labiatae from Pakistan. Pakistan Journal of Botany, 35(5), 671–693.
Popova, M.P., Bankova, V.S., Bogdanov, S., Tsvetkova, I., Naydenski, C., Marcazzan, G.L., & Sabatini, A.G. (2007). Chemical characteristics of poplar type propolis of different geographic origin. Apidologie, 38(3), 306-311. https://doi.org/10.1051/apido:2007013
Popova, M., Trusheva, B., & Bankova, V. (2017). Content of biologically active compounds in Bulgarian propolis: a basis for its standardization. Bulgarian Chemical Communications, 49(2017), 115-120.
Roman, A., Madras-Majewska, B., & Popiela-Pleban, E. (2011). Comparative study of selected toxic elements in propolis and honey. Journal of Apicultural Science, 55(2), 97-106.
Saelao, P., Borba, R.S., Ricigliano, V., Spivak, M., & Simone-Finstrom, M. (2020). Honeybee microbiome is stabilized in the presence of propolis. Biology letters, 16(5), 1-5. https://doi.org/10.1098/rsbl.2020.0003
Sarıkaya, A.O., Ulusoy, E., Öztürk, N., Tuncel, M., & Kolayli, S. (2009). Antioxidant activity and phenolic acid constituents of chestnut (Castania sativa Mill.) honey and propolis. Journal of food biochemistry, 33(4), 470-481. https://doi.org/10.1111/j.1745-4514.2009.00231.x
Sawicka, D., Car, H., Borawska, M.H., & Nikliński, J. (2012). The anticancer activity of propolis. Folia Histochemica et Cytobiologica, 50(1), 25-37.
Sforcin, J.M., Fernandes J.A., Lopes, C.A.M., Bankova, V., & Funari, S.R.C. (2000). Seasonal effect on Brazilian propolis antibacterial activity. Journal of ethnopharmacology, 73(1-2), 243-249. https://doi.org/10.1016/S0378-8741(00)00320-2
Sforcin, J.M. (2016). Biological properties and the rapeuticapplications of propolis. Phytotherapy Research, 30(6), 894-905. https://doi.org/10.1002/ptr.5605
Simone-Finstrom, M., Borba, R.S., Wilson, M., & Spivak, M. (2017). Propolis counteracts some threats to honey bee health. Insects, 8(2), 46. https://doi.org/10.3390/insects8020046
Sobočanec, S., Balog, T., Šariċ, A., Mačak-Šafranko, Ž., Štroser, M., Žarković, K., ..., & Marotti, T. (2011). Antitumor effect of Croatian propolis as a consequence of diverse sex-related dihydropyrimidine dehydrogenase (DPD) protein expression. Phytomedicine, 18(10), 852-858. https://doi.org/10.1016/j.phymed.2011.01.015
Su, L., Yin, J.J., Charles, D., Zhou, K., Moore, J., & Yu, L.L. (2007). Total phenolic contents, chelating capacities, and radical-scavenging properties of black peppercorn, nutmeg, rosehip, cinnamon and oregano leaf. Food chemistry, 100(3), 990-997. https://doi.org/10.1016/j.foodchem.2005.10.058
Şenoğlu Fenerci B. (2021). Eskiçağ’da bal. Masrop E-Dergi, 15(1), 62-74.
Tylkowski, B., Trusheva, B., Bankova, V., Giamberini, M., Peev, G., & Nikolova, A. (2010). Extraction of biologically active compounds from propolis and concentration of extract by nanofiltration. Journal of Membrane Science, 348(1–2), 124–130. http://doi.org/10.1016/j.memsci.2009.10.049
Yüksel, U., Tegin, I., & Ziyadanogullari, R. (2017). Recovery of copper and cobalt from copper slags as selective. Journal of Environmental Science and Engineering A, 6(8), 388-394.
Üreten, H., (2011). Eski Anadolu'da arı ve bal. International journal of history, 3(3), 363-382.