Sığırlarda Beyin, Ak Madde ve Gri Maddenin Cavalieri Prensibi ile Hacim Hesaplamaları

Bu çalışmada sığır beynindeki gri ve beyaz maddenin hacim değerlerini, oranları ve beyin ağırlığı ile volumetrik değerleri arasındaki ilişkiyi hesapladık. Holstein-Friesian ırkı sağlıklı sığıra ait (17-20 aylık) altı taze beyin kullanıldı. Beynin taze ağırlığı dijital tartım kullanılarak ölçüldü. Cavalieri ilkesini kullanarak gri ve beyaz madde hacimlerinin ölçümünden önce beyinler daldırma yöntemi ile % 10 formalin solüsyonunda 3 hafta tespit edildi. Beyinler rostral’den kaudal’e doğru salam dilimleme makinesi (Elta GmBH, Roedermark, Almanya) ile ortalama 8.25 mm kalınlıkta seri olarak kesildi ve beyinlerden ortalama 11 kesit elde edildi. Beyin hacmi 348.00 ± 50.48 cm3, gri madde hacmi 175.70 ± 11.11 cm³ ve beyaz madde hacmide 172.30 ± 42.90 cm³ idi. Gri ve beyaz maddenin hacim oranları sırasıyla % 50.98 ve % 49.02' dir. Beyin ağırlığının beyin hacmine oranı 1 idi. Sığır beynindeki gri ve beyaz maddenin oranı insan beynindeki gri ve beyaz cevherinkine benzer, ancak koyunlardan ve atlardan farklıdır.

Anahtar Kelimeler:

noktalı alan metodu, beyin hacmi, ağırlık hacim oranı, morfometri, stereoloji

Estimation of Volume of Ox Brain and Gray and White Matter with Cavalier’s Principle

We estimated the volume values, ratios, and the relationship between brain weight and volumetric values of gray and white matter of the ox brain. Six fresh brains of Holstein-Friesian strain healthy ox (17-20 months old) were used. Fresh weights of the brains were measured using digital weighing. Brains were fixed by immersion method in 10% formalin solution for 3 weeks before measurements of gray and white matter volumes using Cavalieri’s principle. The brains were serially cut using a salami slicer machine (Elta GmBH, Roedermark, Germany) with a mean thickness of 8.25 mm from rostral to caudal end and averages of 11 sections were obtained from the brains. The brain volume was 348.00 ± 50.48 cm3, 175.70 ± 11.11 cm³ in gray matter volume and 172.30 ± 42.90 cm³ in white matter volume. Volume ratios of gray and white matter were 50.98% and 49.02% respectively. The ratio of brain weight to brain volume was 1. The ratio of gray and white matter in the brain of the ox is similar to that of the gray and white matter in the human brain, but that the sheep and horse are different.

Keywords:

point counting method, brain volume, weight volume ratio, morphometry, stereology,

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Anonymous2017. http://www.stereology.info /cavalieri-estimator/; Accession date: 11.10.2017.
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