Işık Mikroskobik Bir Çalışma: Devede Konjunktiva ile İlişkili Lenfoid Doku

Konjunktiva ile iliĢkili lenfoid doku (CALT), gözün en önemli aksesuar bileĢenlerinden biridir. Bu çalıĢma, sağlıklı develerde konjunktiva ile iliĢkili lenfoid dokunun histolojik dağılımını ve karakteristik özelliklerini ıĢık mikroskobu tekniğiyle ortaya koymak amacıyla yapıldı. ÇalıĢmada toplam 5 adet (13-19 yaĢ aralığında) deveye ait alt ve üst göz kapağı kullanıldı. CALT’a ait genel makroskobik görünüm asetik asit uygulamasıyla ortaya konuldu. Tüm develerde CALT’ın en önemli elemanları olan ve soliter ve agregat lenf foliküllerinin varlığı tespit edildi. Foliküllerin üzerlerinin folikülle iliĢkili epitel (FAE) olarak bilinen ve intra epiteliyal lenfositleri barındıran, kadeh hücrelerinin görülmediği ince, yassılaĢmıĢ epitel ile örtülü olduğu görüldü. Buna ek olarak, lenf foliküllerinin germinal merkez, korona, dom bölgesi ve inter foliküler alanlardan meydana geldiği fark edildi. Ġnterfoliküler alanlarda CALT en önemli karakteristik özelliklerinden bir olan, yüksek endotelli venüllerin varlığı ortaya konuldu. Sonuç olarak, deve CALT’ının sahip olduğu belirleyici özellikleriyle diğer mukozal lenfoid dokularla oldukça yüksek benzelikler göterdiği ve oküler savunma mekanizması içerisinde önemli bir rol oynadığı kanısına varıldı.

Anahtar Kelimeler:

CALT, Deve, FAE, lef folikülü, MALT

A Light Microscopic Study: Conjunctiva-Associated Lymphoid Tissue in Camel

Conjunctiva-associated lymphoid tissue (CALT) is one of the most important accessory component of the eye. This study was undertaken to demostrate the histological distribution and characteristic features of CALT in healhty camels using light microscopy technique. A total upper and lower eyelids of 5 (age range, 13-19) camels were investigated. The gross appearance of CALT were revealed by acetic acid application. Fully intact solitary and aggregated lymphoid follicles were observed as members of CALT in all camels. These follicles were covered by a thin, flattened epitehelium called follicular-associated epithelium (FAE) that contained intra epithelial lymphocytes and lacked goblet cells. In addition germinal centers, corona, subepithelial dome region and interfolliculer areas were noticed within the lymphoid follicles. The presence of high endothelial venules (HEV), a highly distinctive feature of CALT, was confirmed in interfollicular areas. We conclude that CALT of camel closely resembles other mucosal lymphoid tissues and may serve as an important member of ocular defense mechanism with its determinative features.

Keywords:

CALT, camel, FAE, lymphoid follicle, MALT,

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