Chemotherapeutics and antibiotics have been widely used in broiler rations to provide economical benefits and improve the welfare of animals. However, the pathogenic bacteria developed resistance to the antibiotics in both animals and human, thus using antibiotics were prohibited due to the increased concerns about the risk. That is why, there has been a great interest on prebiotics which promote the growth factor of limited beneficial microorganisms and suppress the pathogenic bacteria in the digestive system of human and animals. Prebiotics are also defined as undigestable feed and food ingredients, and growth factor for antibiotic alternatives (performance enhancers)