LPS ile Indüklenen Septisemik Koyunlarda Bazı Eser Element Konsantrasyonlarının İncelenmesi

Enfeksiyona karşı düzensiz immünolojik konak yanıtına bağlı meydana gelen organ bozukluğu olarak tanımlanan endotoksemi, koyunlarda yüksek mortalite ve morbidite sonucu ciddi ekonomik kayıplara neden olan bir hastalıktır. Çalışmada septisemi oluşturulmayan sağlıklı (kontrol) koyunlar (n=6) ile septisemi oluşturulan koyunlardan (n=6) alınan plazma ve serum örneklerinde makro (Ca, Mg, Na, K, P) ve mikro (Fe, Cu, Se, Zn) esansiyel element konsantrasyonları belirlendi. Sonuçlara göre Ca, K ve Se konsantrasyonları açısından gruplar arasındaki farklar istatistiksel olarak anlamlıydı (p≤0.05). Septik hastalarda başarılı teşhis ve tedavi, yeni biyobelirteç belirlenebilmesi ve mekanizmalarının açıklanabilmesi açısından ICP-MS gibi hassas analiz yöntemlerinin kullanıldığı elementler konsantrasyonları ve değişimleri üzerine kapsamlı çalışmalara ihtiyaç duyulmaktadır.

Anahtar Kelimeler:

ICP-MS, Serum.

Investigation of the Concentrations of Some Essential Elements in LPS-Induced Septicemic Sheep

Endotoxemia, which is defined as an organ disorder due to irregular immunological host response to infection, is a disease that causes serious economic losses as a result of high mortality and morbidity in sheep. In the study, macro (Ca, Mg, Na, K, P) and micro (Fe, Cu, Se, Zn) essential element concentrations of plasma and serum samples; taken from healthy (control) (n=6) and septicemic sheep (n=6) were determined. According to the results, the differences between the groups in terms of Ca, K, and Se concentrations were statistically significant (p≤0.05). Comprehensive studies on the concentrations and changes of elements using sensitive analysis methods such as ICP-MS are needed to successful diagnose and treat septic patients, identify new biomarkers, and explain their mechanisms.

Keywords:

Septicemia, ICP-MS, Macro element, Micro element Plasma, Serum.,

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