Abdullah ERYAVUZ, İbrahim DURMUŞ, İsmail KÜÇÜKKURT, Sinan İNCE

Koyunlarda Yeme Yucca Schidigera ve Zeolit Katılmasının Kan Sitokin ve Tiroid Hormon Düzeylerine Etkileri

Bu çalışmanın amacı, koyunlarda yeme steroidal saponin içeren Yucca schidigera (YS) ve zeolit kaynağı olarak klinoptilolitin birlikte ilavesinin kan IL-1β, IL-6, TNF-α ve tiroid hormon düzeylerine etkilerini karşılaştırmaktır. Çalışmada, toplam 24 koyun kullanıldı ve koyunlar her biri 6 hayvan içeren Kontrol, YS, Zeolit ve YS + Zeolit olmak üzere dört gruba ayrıldı.Kontrol grubundaki hayvanlar temel yemle beslenirlerken, YS gruptaki hayvanlar yeme 1500 ppm düzeyinde YS ilave edilmiş yemle, Zeolit gruptaki hayvanlar %3 düzeyinde klinoptilolit ilave edilmiş yemle ve YS+Zeolit gruptakiler ise 1500 ppm YS ile %3 klinoptilolit ilave edilmiş yemle beslendiler. Toplam 30 gün süren deneme döneminin sonunda, hayvanlardan kan örnekleri alındı. IL-1β, IL-6, TNF-α, T3 ve T4'ün plazma düzeyleri, ticari kitler kullanılarak ELİSA cihazında belirlendi. Yeme YS ilavesi plazma IL-1β düzeiyini azalttırken, YS+Zeolitin birlikte yeme ilavesi ise plazma TNF-α düzeyini düşürdü. Buna karşın, çalışmada, uygulamaların IL-6, T3 ve T4 düzeylerine etkisinin olmadığı tespit edildi. Sonuç olarak, iki doğal yem katkı maddesi olan zeolit ve YS’nın yeme ayrı ayrı katılması yerine birlikte ilavesinin koyun kanında proinflamatuar sitokinlerin üretimini etkileyebileceği kanaatine varıldı.

Anahtar Kelimeler:

zeolit, sitokinler, tiroid hormonları, koyun, saponinler

Effects of Yucca Schidigera and Zeolite Supplementation to Diet on Blood Cytokines and Thyroid Hormones Concentrations in Sheep

This study is aimed to compare the effects of combined supplementation of Yucca schidigera (YS) and clinoptilolite as a zeolite source to diet on the blood IL-1β, IL-6 and TNF-α and thyroid hormones concentrations in sheep. A total of 24 sheep were divided four groups as the Control, YS group, Zeolite and YS + Zeolite group and each group was contained 6 animals. The basal diet was given to animals in control group, while the animals in the Zeolite, YS and YS + Zeolite groups were fed with the experimental diet contained the basal diet plus 3% clinoptilolite, 1500 ppm YS and 1500 ppm YS + 3% clinoptilolite, respectively. The blood samples were taken from the animals in the end of experimental period lasted 30 days. The plasma IL-1β, IL-6, TNF-α, T3 and T4 concentrations were determined by using the commercial ELISA. The supplementation of YS decreased the plasma IL-1β concentration and the combined supplementation of Zeolite and YS lowered the plasma TNF-α concentration. The plasma IL-6, T3 and T4 concentrations were not affected by the treatments. As a result, it was concluded that the combined supplementation of zeolite and YS, which are two natural feed additives, instead of adding them separately to the feed, may affect the proinflammatory cytokines production in sheep blood.

Keywords:

zeolite, cytokines, Thyroid hormones, sheep, saponins,

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