Fatih AVDATEK, Ruhi TÜRKMEN, Hasan Hüseyin DEMİREL, Yavuz Osman BİRDANE

Glifosat Bazlı Herbisite Maruz Kalan Sıçanlarda N-Asetilsisteinin Testis Oksidatif Hasarı, Spermatolojik Parametreler ve DNA Hasarı Üzerindeki Koruyucu Etkisi

Bu çalışmanın amacı, N-asetilsisteinin (NAC) sıçanlarda Glifosat (GLF) 'nin neden olduğu testiküler oksidatif hasar, spermatolojik parametreler ve DNA hasarı üzerindeki koruyucu etkisini incelemektir. Toplam yirmi sekiz Wistar erkek sıçan, eşit bir şekilde dört gruba ayrılarak değerlendirildi. Kontrol grubunu olan grup I'deki sıçanlar, GLF veya NAC olmaksızın normal diyetle beslendiler, grup II’deki hayvanlara 160 mg / kg / günlük NAC içeren normal rat diyeti uygulandı, grup III’deki hayvanlara 375 mg / kg / günlük GLF içeren normal rat diyeti verildi ve grup IV’deki hayvanlara 160 mg / kg / günlük NAC + 375 mg / kg / günlük GLF içeren normal rat diyeti uygulandı. GLF uygulaması sıçanların testis dokusunda spermatozoon motilitesini, anormal spermatozoon oranını, spermatozoon plazma membran bütünlüğünü, glutatyon ve süperoksit dismutaz düzeyini azaltmıştır. Diğer taraftan GLF uygulanan grupta yüksek malondialdehid düzeyi ve DNA hasarı saptandı. Ayrıca histopatolojik olarak testis dokusunda spermatozoon konsantrasyonunda azalma ve sertoli hücrelerin dejenerasyonu belirlendi. NAC ve NAC + GLF uygulaması, lipid peroksidasyonunu ve GLF tarafından indüklenen DNA hasarını, sıçanların testislerinde antioksidan enzimlerin aktivitesini ve hücre bütünlüğünü tersine çevirdi. Yukarıda belirtilen bulgular, NAC'nin GLF'nin neden olduğu lipit peroksidasyonunu azalttığını, antioksidan savunma mekanizmasını geliştirdiğini ve sıçanların testislerinde doku hasarını rejenere ettiğini göstermektedir.

Anahtar Kelimeler:

N-asetilsistesin, Glifosat, Rat spermatozoon, DNA hasarı

Protective Effect of N-Acetylcysteine on Testicular Oxidative Damage, Spermatological Parameters and DNA Damage in Glyphosate-Based Herbicide-Exposed Rats

The aim of this study was to examine the protective effect of N-acetylcysteine (NAC) on testicular oxidative damage, spermatological parameters and DNA damage caused by Glyphosate (GLF) in rats. In total, twenty-eight Wistar male rats were evaluated by being separated into four groups in an equal way. Rats in group I, which represented the control group, were fed normal diet without GLF or NAC, group II received normal feed containing 160 mg/kg/daily NAC, group III received normal feed containing 375 mg/kg/daily GLF, and group IV received normal feed containing 160 mg/kg/daily NAC + 375 mg/kg/daily GLF. GLF administration decreased sperm motility, abnormal sperm rate, sperm plasma membrane integrity, glutathione level and superoxide dismutase in the rats’ testicular tissue. On the other hand, high malondialdehyde level and DNA damage were detected in the group administered with GLF. Besides, in histopathological terms, a decrease in sperm concentration and degeneration of sertoli cells were determined in the testicular tissue. NAC and NAC+GLF administration reversed lipid peroxidation and DNA damage induced by GLF, the activity of antioxidant enzymes and cell integrity in rats’ testis. The above-mentioned findings indicate that NAC reduces lipid peroxidation caused by GLF, improves the antioxidant defense mechanism and regenerates tissue damage in rats’ testis.

Keywords:

N-acetylcysteine, Glyphosate, Rat sperm, DNA damage,

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