Hüseyin ÖZKAN, İrem KARAASLAN, Ufuk KAYA, Sevda DALKIRAN, İbrahim ALAKUŞ, Baran ÇAMDEVİREN, Hasan Hüseyin KEÇELİ, Akın YAKAN

Gıda Kısıtlaması Yapılan Ratlarda Karaciğerde Bazı Lipojenik Genlerin İfade Düzeyleri Ve Yağ Asidi Profili

Bu çalışma gıda kısıtlamasının karaciğerdeki yağ asidi profili ve majör lipojenik genlerin ekspresyon seviyeleri üzerindeki etkilerini belirlemeyi amaçlamıştır. 16 adet Wistar albino rat iki gruba ayrılarak 4 hafta boyunca farklı diyetlerle beslenmiştir. Birinci grup (Kontrol grubu) ad libitum beslenirken, diğer gruba günlük ihtiyacın yarısı (Yem Kısıtlama grubu, YK) kadar yem verilmiştir. Haftalık besin tüketimi ve vücut ağırlığı değişimlerinin yanı sıra beslenme periyodu sonrasında total kolesterol, HDL, LDL ve trigliserit düzeyleri belirlenmiştir. Yağ asidi profiline ek olarak karaciğerde FASN ve SCD-1 genlerinin ekspresyon seviyeleri ölçülmüştür. FR’nin ortalama vücut ağırlığı 7. günden sonra azalmaya başlarken, YK'de plazma glukoz seviyeleri kontrol grubuna kıyasla daha düşük bulunmuştur. YK grubunda FASN geni yaklaşık 6 kat artarken (P<0,05), SCD-1 önemsiz olacak şekilde yaklaşık 3 kat artmıştır. FR grubunda C15:0, C18:1 n9 trans, C18:2 n6 cis, C21:0, C20:2, C20:5 n3, n6 ve UFA miktarı kontrole göre daha düşükken, C16:0, C18:2 n6 trans, C20:3 n6, C22:6 n3, C22:1 n9, C22:2 ve SFA miktarının daha yüksek olduğu belirlenmiştir. Besin kısıtlamasının, maruz kalma süresi ve oranının dikkate alınmasına ek olarak, diğer metabolik organlardaki moleküler aktivite ve etkileşimler araştırılmalıdır.

Anahtar Kelimeler:

FASN, karaciğer, SCD-1, yağ asitleri, yem kısıtlama

Expression Patterns of Some Lipogenic Genes and Fatty Acid Profile of Liver in Food-Restricted Rats

This study aimed to determine food restriction effects on the profile of fatty acids and major genes on lipogenesis expressions in liver. 16 Wistar albino rats were divided into two groups and different diets were given to groups for 4-weeks. First group was fed ad libitum (Control group), another group was fed the half amount of the daily requirement (Food Restriction group, FR). As well as weekly food consumption and body weight changes, total cholesterol, HDL, LDL, and triglyceride levels were determined at the end of the feeding period. In addition to the fatty acid profile, FASN and SCD-1 genes expression levels were measured in the liver. While the body weight averages decreased after 7 days and remained similar, plasma glucose levels were found lower in the FR. FASN was upregulated approximately 6 folds, and SCD-1 increased insignificantly about 3 folds in the FR. C15:0, C18:1 n9 trans, C18:2 n6 cis, C21:0, C20:2, C20:5 n3, n6 and UFA were lower, while C16:0, C18:2 n6 trans, C20:3 n6, C22:6 n3, C22:1 n9, C22:2 and SFA were higher in FR. In addition to considering the exposure time and rate of food restriction, molecular activity and interactions in other metabolic organs should be investigated.

Keywords:

FASN, fatty acids, food restiction, liver, SCD-1,

Tam Metin

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Bibtex	@araştırma makalesi { kvj1173180, journal = {Kocatepe Veterinary Journal}, issn = {1308-1594}, eissn = {2147-6853}, address = {}, publisher = {Afyon Kocatepe Üniversitesi}, year = {2023}, volume = {16}, number = {2}, pages = {131 - 142}, doi = {10.30607/kvj.1173180}, title = {Expression Patterns of Some Lipogenic Genes and Fatty Acid Profile of Liver in Food-Restricted Rats}, key = {cite}, author = {Özkan, Hüseyin and Karaaslan, İrem and Kaya, Ufuk and Dalkıran, Sevda and Alakuş, İbrahim and Çamdeviren, Baran and Keçeli, Hasan Hüseyin and Yakan, Akın} }
APA	Özkan, H. , Karaaslan, İ. , Kaya, U. , Dalkıran, S. , Alakuş, İ. , Çamdeviren, B. , Keçeli, H. H. & Yakan, A. (2023). Expression Patterns of Some Lipogenic Genes and Fatty Acid Profile of Liver in Food-Restricted Rats . Kocatepe Veterinary Journal , 16 (2) , 131-142 . DOI: 10.30607/kvj.1173180
MLA	Özkan, H. , Karaaslan, İ. , Kaya, U. , Dalkıran, S. , Alakuş, İ. , Çamdeviren, B. , Keçeli, H. H. , Yakan, A. "Expression Patterns of Some Lipogenic Genes and Fatty Acid Profile of Liver in Food-Restricted Rats" . Kocatepe Veterinary Journal 16 (2023 ): 131-142 <
Chicago	Özkan, H. , Karaaslan, İ. , Kaya, U. , Dalkıran, S. , Alakuş, İ. , Çamdeviren, B. , Keçeli, H. H. , Yakan, A. "Expression Patterns of Some Lipogenic Genes and Fatty Acid Profile of Liver in Food-Restricted Rats". Kocatepe Veterinary Journal 16 (2023 ): 131-142
RIS	TY - JOUR T1 - Gıda Kısıtlaması Yapılan Ratlarda Karaciğerde Bazı Lipojenik Genlerin İfade Düzeyleri Ve Yağ Asidi Profili AU - HüseyinÖzkan, İremKaraaslan, UfukKaya, SevdaDalkıran, İbrahimAlakuş, BaranÇamdeviren, Hasan HüseyinKeçeli, AkınYakan Y1 - 2023 PY - 2023 N1 - doi: 10.30607/kvj.1173180 DO - 10.30607/kvj.1173180 T2 - Kocatepe Veterinary Journal JF - Journal JO - JOR SP - 131 EP - 142 VL - 16 IS - 2 SN - 1308-1594-2147-6853 M3 - doi: 10.30607/kvj.1173180 UR - Y2 - 2023 ER -
EndNote	%0 Kocatepe Veteriner Dergisi Expression Patterns of Some Lipogenic Genes and Fatty Acid Profile of Liver in Food-Restricted Rats %A Hüseyin Özkan , İrem Karaaslan , Ufuk Kaya , Sevda Dalkıran , İbrahim Alakuş , Baran Çamdeviren , Hasan Hüseyin Keçeli , Akın Yakan %T Expression Patterns of Some Lipogenic Genes and Fatty Acid Profile of Liver in Food-Restricted Rats %D 2023 %J Kocatepe Veterinary Journal %P 1308-1594-2147-6853 %V 16 %N 2 %R doi: 10.30607/kvj.1173180 %U 10.30607/kvj.1173180
ISNAD	Özkan, Hüseyin , Karaaslan, İrem , Kaya, Ufuk , Dalkıran, Sevda , Alakuş, İbrahim , Çamdeviren, Baran , Keçeli, Hasan Hüseyin , Yakan, Akın . "Expression Patterns of Some Lipogenic Genes and Fatty Acid Profile of Liver in Food-Restricted Rats". Kocatepe Veterinary Journal 16 / 2 (Haziran 2023): 131-142 .
AMA	Özkan H. , Karaaslan İ. , Kaya U. , Dalkıran S. , Alakuş İ. , Çamdeviren B. , Keçeli H. H. , Yakan A. Expression Patterns of Some Lipogenic Genes and Fatty Acid Profile of Liver in Food-Restricted Rats. Kocatepe Veterinary Journal. 2023; 16(2): 131-142.
Vancouver	Özkan H. , Karaaslan İ. , Kaya U. , Dalkıran S. , Alakuş İ. , Çamdeviren B. , Keçeli H. H. , Yakan A. Expression Patterns of Some Lipogenic Genes and Fatty Acid Profile of Liver in Food-Restricted Rats. Kocatepe Veterinary Journal. 2023; 16(2): 131-142.
IEEE	H. Özkan , İ. Karaaslan , U. Kaya , S. Dalkıran , İ. Alakuş , B. Çamdeviren , H. H. Keçeli ve A. Yakan , "Expression Patterns of Some Lipogenic Genes and Fatty Acid Profile of Liver in Food-Restricted Rats", , c. 16, sayı. 2, ss. 131-142, Haz. 2023, doi:10.30607/kvj.1173180