Mahmut Şerif YILDIRIM, Ali Rıza TÜMER, Ramazan AKÇAN, Burak OMAY, Deniz AKATA, İlyas ONBAŞILAR

SIÇANLARDA KARACİĞER VE ÇİZGİLİ KAS BİLGİSAYARLI TOMOGRAFİ ATENÜASYONLARI İLE POST-MORTEM İNTERVAL TAYİNİ

AMAÇ: Post-mortem interval (PMI) tayinini hedefleyen ölüm sonrası bilgisayarlı tomografik çalışmalara ilgi artmaktadır. Literatürde çoğu çalışma, kontrolsüz veya nispeten daha az kontrollü bir durumda yürütülmüştür. Bu çalışma, sıkı kontrollü çevresel koşullar altında PMI tahmininde karaciğer ve çizgili kasların bilgisayarlı tomografi atenüasyonlarının kullanılabilirliğini araştırmaktadır. GEREÇ VE YÖNTEM: 30 Wistar Albino sıçanın ölümden sonraki ilk 30 dakikada ve post-mortem 12, 24, 36, 48, 72, 96, 120, 144 ve 168. saatte bilgisayarlı tomografi görüntüleri alındı. 0,12 cm2 ilgi alanında karaciğerin lateral lobları ve paraspinal kasın tekrarlayan atenüasyonları kaydedildi. BULGULAR: Karaciğer ve paraspinal kasların atenüasyon değerleri, ölümden 12 saat sonra daha yüksek bulundu. Hem erkek (p=0,01) hem de dişi sıçanlarda (p=0,01) otopsiden 30 dakika sonra elde edilen görüntüler ile otopsiden 12 saat sonra elde edilen görüntüler arasında anlamlı fark gözlendi. PMI tayini için karaciğer ve kas dokusu için farklı atenüasyon eşik değerleri belirlendi." SONUÇ: Önerilen model ile karaciğer ve çizgili kasların atenüasyon değerlerinin kullanımı, PMI tayini için faydalı bulunmuştur. Atenüasyonun eşik değerleri, özellikle karaciğer için, PMI sınırlandırılmasında kullanılabileceği anlaşılmıştır.

Anahtar Kelimeler:

Post-mortem interval tayini, Post-mortem bilgisayarlı tomografi, Karaciğer atenüasyonu, Kas atenüasyonu

POST-MORTEM INTERVAL ESTIMATION BASED ON LIVER AND STRIATED MUSCLE COMPUTED TOMOGRAPHY ATTENUATIONS IN RATS

OBJECTIVE: The interest on post-mortem computed tomographic studies targeting post-mortem interval (PMI) estimations is increasing. Most studies have been conducted in an uncontrolled or relatively less controlled condition. However, this study investigates the usefulness of computed tomography attenuations of the liver and striated muscles in PMI estimation under strictly controlled environmental conditions. MATERIAL AND METHODS: Post-mortem computed tomography images of 30 Wistar Albino rats were obtained in the first 30 min after death and 12, 24, 36, 48, 72, 96, 120, 144 and 168-hours following death. Repeated attenuations of both thelateral lobes of the liver and paraspinal muscle were recorded in 0.12-cm2 regions of interest. RESULTS: The attenuation values of the liver and paraspinal muscles were higher 12-hour post-mortem. A significant difference was observed between the images obtained 30 minutes post-mortem and those obtained 12 hours post-mortem in both male (p=0.01) and female rats (p=0.01). Different cut-off attenuation values for the liver and muscle tissue were determined to estimate the post-mortem interval. CONCLUSIONS: The use of attenuation values of the liver and striated muscles with the proposed model was found to be beneficial for the determination of PMI. The cut-off values of attenuation, especially for the liver, can be used in the delimitation of the post-mortem interval.

Keywords:

Post-mortem interval estimation, Post-mortem computed tomography, Liver attenuation, Muscle attenuation,

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