Sedef Zeliha ÖNER, İlknur KALELİ, Babür KALELİ

Vajinal Mikrobiyal Türlerin Bakteriyel Vajinozis ve Human Papillomavirus ile İlişkisinin Araştırılması

Amaç: Bu çalışmada vajinal mikrobiyal bakteri türlerinin bakteriyel vajinozis (BV) ve yüksek riskli Human papillomavirus (HR-HPV) ile ilişkisinin araştırılması amaçlanmıştır.Yöntem: Çalışmaya 121 kadın dahil edilmiştir. BV tanısı için Gram boyama yapıldı ve Nugent skoruna göre değerlendirildi. HR-HPV tespiti Real Time High Risk HPV kiti kullanılarak real-time PCR yöntemiyle gerçekleştirildi. Vajinal mikrobiyal türler ve Gardnerella vaginalis subtipleri de real-time PCR ile saptanmıştır.Bulgular: Bakteriyel vajinozis görülme oranı %38,8 olarak bulunmuştur. Tür sayısı ortalaması; bakteriyel vajinoziste, BV ara değer ve BV negatif örneklere göre anlamlı olarak yüksek görülmüştür. (p=0,001). BVAB2 (p=0,043), Provetella spp. (p=0.015), Leptotrichia/Sneathia (p=0,001), Megaspheara (p=0,048), BV ile ilişkili olduğu görülmüştür. Rastgele seçilen 50 örnekte G.vaginalis subtipleri değerlendirilmiştir. En yaygın “clade 4” subtipi tespit edilmiştir. HR-HPV prevalansı %9,9 olarak saptanmıştır. En sık saptanan HR-HPV tipi HPV 16 %58,33’dü. HR-HPV pozitif ve negatif hastalarda Lactobacillus sp. ortalaması arasında anlamlı fark görülmemiştir (p=0.23). Belirlenen türler ile HR-HPV pozitifliği arasında ilişki saptanmamıştır (p=0,436). Sonuç: BV tespit edilen kadınların vajenlerinde bakteri çeşitliliği daha fazlaydı. Bakteriyal vajinozis ile Megaspheara, BVAB2, Provetella spp. ve Leptotrichia/Sneathia’nın ilişkili olduğu bulunmuştur.

Anahtar Kelimeler:

vajinal mikrobiyal türler, bakteriyel vajinozis, Gardnerella vaginalis, insan papillomavirüs

An Investigation the Relationship of Vaginal Microbial Bacteria Species with Bacterial Vaginosis and High-Risk Human Papillomavirus

Objective: This study was aim to investigate the relationship of vaginal bacterial species with High-Risk Human Papillomavirus (HR-HPV) and bacterial vaginosis (BV).Methods: One hundred and twenty-one women were included in the study. Gram stain was performed for the diagnosis of BV and evaluated according to the Nugent score. HR-HPV diagnosis was made by real-time PCR . Detection of vaginal microbial species and Gardnerella vaginalis subtypes were also performed by real-time PCR.Results: The prevalence of BV was found as 38.8%. The mean number of species was found significantly higher in BV-positive samples compared to BV-intermediate and BV-negative samples (p=0.001). Lactobacillus iners (p=0.036), BVAB2 (p=0.043), Provetella spp. (p=0.015), Leptotrichia/Sneathia (p=0.001), Megaspheara (p=0.048) were found to be associated with bacterial vaginosis. Gardnerella vaginalis subtypes were evaluated in 50 randomly selected samples. The most common strain that was found was “clade 4”. The prevalence of HR-HPV was 9.9%. HPV 16 was the most common HR-HPV type (58.3%). There was no significant difference between the mean value of Lactobacillus sp. HR-HPV-positive and negative samples (p=0.23). No association was found between the specified species and HR-HPV-positive samples (p=0.436).Conclusion: Bacterial diversity was greater in BV-positive patients and BV was significantly associated with Lactobacillus iners, Megaspheara, BVAB2, Provetella spp. and Leptotrichia / Sneathia.

Keywords:

bacterial vaginosis, Gardnerella vaginalis, Human papillomavirus, vaginal microbial species,

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