Buket Demirci, İmran Kurt Ömürlü, Ayşe İpek Akyüz Ünsal, Harun Çakmak, İbrahim Meteoğlu, Ceren Günel, Tolga Kocatürk

DENEYSEL ALERJİK KONJONKTİVİT MODELİNDE ÇÖREK OTU BİLEŞENLERİNDEN TİMOKİNON’UN ETKİSİ

Amaç: Alerjik konjonktivit tedavisi güç bir hastalıktır; yeni tedavi seçeneklerine ihtiyaç vardır. Bu çalışmada anti-inflamatuar ve anti-alerjiközellikleri olan ve çörek otundan (Nigella Sativa) elde edilen timokinonun (TK) sıçan alerjik konjonktivit (AK) modeli üzerindeki etkisininhistolojik yöntemlerle araştırılması amaçlanmıştır.Yöntem: Çalışmaya 48 adet 4-6 aylık yetişkin Wistar Albino dişi sıçan dahil edildi. Sıçanlar; kontrol, AK, AK+Deksametazon, 10 mg/kg TK,AK+3 mg/kg TK ve AK+10 mg/kg TK şeklinde rastgele 6 gruba ayrıldı. AK geliştirmek için, 30 mg/ml adjuvan Al(OH)3 ve 0,3 mg Ovalbumin,serum fizyolojik içinde her sıçana 1 mL intraperitoneal (i.p.) olarak gün aşırı 7 kez uygulandı. Ardı sıra 15-21. günlerde doğrudan konjonktivaüzerine serum fizyolojik içinde %2'lik Ovalbumin 7 kez damlatıldı. Ait olduğu gruba göre TK ilgili dozlarda i.p. olarak uygulandı. Konjonktivadokusu mast hücre sayıları (MHS) ile histopatolojik ve immünohistokimyasal MCT, TGFβ ve TNFα boyama sonuçları istatistiksel olarakdeğerlendirildi.Bulgular: Timokinonun tek başına sistemik uygulanması konjonktiva histolojisinde değişikliğe neden olmadı. Ovalbüminle duyarlı hale getirilenAK grubu kontrol grubuyla karşılaştırıldığında, konjonktiva epitelindeki MHS istatistiksel olarak anlamlı artış gösterdi (p

THE EFFECT OF THYMOQUINONE ON EXPERIMENTAL MODEL OF ALLERGIC CONJUNCTIVITIS

Objective: Treatment of allergic conjunctivitis is challenging and new treatment modalities are required. In this study; the effect of Thymoquinone (TK), a natural derivative of Nigella Sativa with its anti-allergic and anti-inflammatory properties, was investigated on an experimental rat model of allergic conjunctivitis (AK). Methods: Forty-eight female Wistar Albino rats aging between 4-6 months were included in this study. Rats were divided into 6 groups as following: control, AK, AK + Dexamethasone, 10 mg/kg TK, AK + 3 mg/kg TK and AK + 10 mg/kg TK. To induce an AK rat model, Ovalbumin (0.3 mg) and Al(OH)3 (30 mg/ml) in 1 ml of %0.9 NaCl were given 7 times intraperitoneally (i.p.) to each rat every other day. Ovalbumin drops (2%) were installed everyday between 15 th -21th days directly on conjunctiva. TK was applied i.p. with above mentioned doses. Tissue Mast Cell Counts (MCC) and histopathological - immunohistochemical staining results were statistically evaluated. Results: Systemic administration of Thymoquinone alone did not demonstrate any detrimental effect on eye tissues. MCC of Ovallbumin sensitized AK group was significantly higher than the control cases (p

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