The Comparision between the Modified Auramine Rhodamine Flourochrome Staining and Ziehl-Neelsen Staining Method from Sputum Specimens in the Presence with Radiometric Bactec Tb System

Amaç: Akciğer tüberkülozunun teşhisi balgamın bakteriyolojik tetkiki ile mümkündür. Balgam yaymaların mikroskobik incelemesi etkilidir ve hastalığı doğrular. Fakat Balgam yaymaların mikroskobik incelemesinin duyarlılığı düşüktür. Çalışmamızda balgamdan Direkt yaymayla (DS) yapılan preparatların Modifiye– auramine flourokrom boyama (MR-AFS) yöntemiyle aside dirençli basillerin (AFB) boyanmasının kullanışlı olduğunu göstermeyi amaçladık. Hastalar ve Yöntem: Toplam 218 hastadan 10 pozitif kontrol ve 10 negatif kontrolden alınan AFB’in MR-AFS ve Ziehl-Neelsen Boyama ZNS yöntemleri ile BACTEC TB sistem altın standard olarak kabul edilerek incelendi ve kıyaslandılar. Bulgular: BACTEC TB sistemi ile 16 örnek, 13 örnek MR-AFS yöntemi ile ve 11 örnek ZNS AFS yöntemi ile pozitif olarak tespit edilmiştir. Pozitif olarak tespit edilen örneklerin oranları ZNS yönteminde MR-AFS yöntemine gore belirgin olarak yüksek olarak bulunmuştur (p < 0.01). BACTEC TB sistem altın standard olarak kabul edildiğinde MR-AFS’nin sensitivite ve spesivitesi sırasıyla %84 ve %100, ZNS’nin sensitivite ve spesivitesi sırasıyla %68.8 ve %100 olarak tespit edilmiştir. Sonuç: Klinik örneklerden AFB araştırılmasında MR-AFS yönteminin ZNS yöntemine göre daha hızlı pratik ve güvenli olduğu tespit edilmiştir. Ayrıca gelişmekte olan floresan mikroskobunun olduğu ülkelerde MR-AFS’nin kullanışlı bir yöntemdir

Pages 24-29 The Comparision between the Modified Auramine Rhodamine Flourochrome Staining and Ziehl-Neelsen Staining Method from Sputum Specimens in the Presence with Radiometric Bactec Tb System

-
Keywords:

-,

___

  • Horwitz MA, Lee B_WE, Dillon BJ, Harth G. Protective immunity against tuberculosis induced by vaccination with major extra cellular proteins of Mycobacterium tuberculosis, Proc Natl Acad Sci 1995; 92: 1530-4
  • Nolte FS, Metchock B. Mycobacterium. In: Muray PR, Baron EJ, Pfaler MA, Tenover FC, Yolken RH (eds.), Manual of Clinical Microbiology, 6th ed. Washington, DC: ASM Press 1966: pp. 400-37
  • Colston J. Tuberculosis: The Return of the Great White bin/html-check.pl
  • Pao CC, Yan TSB, You JB, Maa JS, Fiss EH, Chang CH. Detection and identification of Mycobacterium amplification, J Clin Microbiol 1990; 28: 1877-80 by DNA
  • Middlebrook, G, Reggiordo Z Tigett WD. Automatable radiometric detection of growth of Mycobacterium tuberculosis in selective media, Am Rev Respis Dis 1977; 115: 1066
  • Siddiqi, SH. 1989 Bactec T.B. System Product and Procedure Manual Revision B. Becton Dickinson Diagnostic Instruments System Towson, Md.
  • Cserni G. Auramine fluorescence for acid-fast bacilli in formalin-fixed paraffin-embedded tissues, Am J Clin Pathol 1995; 103 114
  • Ellis RC, Zabrowarny LA. Safer staining method for acid-fast bacilli, J Clin Pathol 1995; 103: 114 9. Kent Mycobacteriology: A guide for the level III laboratory. Centers for Disease Control, U.S. Department of Health and Human Services, Atlanta, 1985 Public Health
  • Kuper SWA, May JR. Detection of acid-fast organisms in tissue sections by fluorescence microscopy, J Pathol Bacteriol 1960; 79: 59-67
  • Bencroft JD. Stevens A. Theory and Practice of Histological Techniques, MBBS MRC Pach Edinburgh London & New York 1977
  • Bradburgy P, Gordon KC. Connective tissues and stains. İn: Bancroft JD, 3rd Edition The Bath Press Avon Great Britain, 1990: pp. 132
  • Epi Info Version 6.0, Public Domain Software for Epidemiology and Disease Surveillance, Centers for Disease Control Epidemiology Programme Office Atlanta, Georgia July 1991
  • Gordin F, Slutkin G. The validity of acid-fast smears in the diagnosis of pulmonary tuberculosis, Arch Pathol Lab Med 1990; 114, 1025-1027
  • Cook SH, Partos RE, Piersen CL, Frank TS. Detection mycobacteria by the polymerase chain reaction, Diagn Mol Pathol 1994; 3: 53-58 of atypical
  • Deshmukh SR, Mantri SB, Kendre PB, Nagoba BS. A comparison of sputum examination for acid fast bacilli by modified Schaeffer & Fulton stain, Ziehl- Neelsen stain & cold stain. Indian J Med Res 1996 Jun;103: 294-295
  • McCarter YS, Robinson A. Detection of acid-fast bacilli in concentrated primary specimen smears stained with Rhodamine–auramine at room temperature and at 37°C, J Clin Microbiol 1994; 32: 2487-2489
  • Zanden AG, Hoentjen AH, Heilmann FG, Weltevreden EF, Schouls LM, Embden JD. Simultaneous detection and strain differentiation of Mycobacterium tuberculosis complex in paraffin wax embedded tissues and in stained microscopic preparations, Mol Pathol 1998; 51(4):209-14
  • Collins FM, Morrison NE, Dhople AM, Watson SR. Mycobacterium suspensions. A comparison of three staining procedures, Int J Lepr Other Mycobact Dis 1980; 48(4):402-407 carried M. tuberculosis leprae and
  • Wöckel W. Auramine fluorescence for acid-fast bacilli tissues. Am J Clin Pathol 1995; 103: 667 paraffin-embedded
  • Rattan A, Kishore K, Singh S, Jaber M, Xess I, Kumar R. Evaluation of a safe sputum processing method for detecting tuberculosis, J Clin Pathol 1994; 47(5): 411-3
  • Githui W, Kitui F, Juma ES, Obwana DO, Mwai J, Kwamanga D. A comparative study on the reliability of the fluorescence microscopy and Ziehl-Neelsen method in the diagnosis of pulmonary tuberculosis, East Afr Med J 1993; 70(5): 263-266
  • Tarhan G, Ordulu L, Gümüşlü F, Ceyhan I, Cesur S Comparison of auramine-rhodamine and Erlich- Ziehl-Neelsen staining methods for the diagnosis of tuberculosis. Mikrobiol Bul 2003;37(2-3):131-136.
  • Tarhan G, Cesur S, Ozyilmaz E, Ceyhan I, Güler M, Konur O, Ciftçi B, Erdoğan Y. Microbiological evaluation of the cases prediagnosed as pulmonary tuberculosis based on clinical and radiological findings Mikrobiyol Bul. 2005;39(4): 431-435.
  • Botis S, Koemoth PP, Correa de Brito JM, Brock M. Growth of some mycobacterial strains in 7H12 Middlebrook liquid medium in the presence of auramine, Rev Ig Bacteriol Virusol Parazitol Epidemiol Pneumoftiziol 1989; 38(2): 137-141
  • Gérôme P, Fabre M, Soler CP, De Pina JJ, Simon F. Comparison of the mycobacteria growth indicator tube with solid culture for the detection of tuberculosis bloodPathol Biol (Paris). 2008; 57(1):44-50.
  • Öztürkeri H, Demir MA, Ballı S, Aydilek R, Kocabeyoğlu Ö, Yenen OŞ. Comparative evaluation of different staining methods in the diagnosis of tuberculosis. Mikrobiyol Bült, 1997; 31: 231-236
  • Anargyros P, Asti DS, Lis IS. Comparison of improved Bactec and Löwenstein – Jensen media for culture of Mycobacteria from clinical specimens. J Clin Micro 1990; 28: 1288-1291
  • Samastı M. Tüberküloz’da mikrobiyolojik tanı yöntemleri. Klimik Derg 1989; 2(1): 6-9
  • Coresponding author: Teoman Zafer Apan
  • Kırıkkale Üniversitesi
  • Department of Microbiology and Clinical
  • Microbiology, Faculty of Medicine Kırıkkale, Türkiye Tel: +9005332235034 Fax: +9003122522625
  • E-mail: teomanapan@gmail.com
Kırıkkale Üniversitesi Tıp Fakültesi Dergisi-Cover
  • ISSN: 2148-9645
  • Yayın Aralığı: Yılda 3 Sayı
  • Başlangıç: 1999
  • Yayıncı: KIRIKKALE ÜNİVERSİTESİ KÜTÜPHANE VE DOKÜMANTASYON BAŞKANLIĞI