Ercan YUVANÇ, Devrim TUĞLU, Üçler KISA, Önder BOZDOĞAN, Ertan BATİSLAM, Erdal YILMAZ

DENEYSEL TESTİS TORSİYONU SONRASI İSKEMİ/REPERFÜSYON HASARINDA İLOPROST VE DÜŞÜK DOZ METOTREKSAT’IN KORUYUCU ETKİSİ

Amaç: Testis torsiyonunda cerrahi olarak detorsiyone edilen testiste gelişen iskemi/reperfüzyon (I/R) hasarı üzerine İloprost ve düşük doz Metotreksat’ın koruyucu etkilerini araştırmak.Gereç ve Yöntem: Ratlar rastgele 4 gruba ayrıldı. Grup 1 (sham), Grup 2 = İskemi/Reperfüzyon, ilaç verilmedi. Grup 3 = İskemi/Reperfüzyon + İloprost (10 µg/kg) ve Grup 4 = İskemi/Reperfüzyon + Düşük doz Metotreksat (6 mg/kg). Torsiyon/iskemi ve detorsiyon/reperfüzyon süreleri 4’er saat olarak belirlenmiştir. Medikasyonlar intraperitoneal yolla testis torsiyonunu takiben 3. saatte verilmiştir. Her 4 grupta işlemin başlangıcından 8 saat sonra detorsiyone sağ testis çıkarılmıştır.Bulgular: Çalışmamızda, iloprost ve düşük doz metotreksat verilmesinin total antioksidan kapasiteyi (TAK) istatistiksel olarak anlamlı arttırdığını, oksidan kapasite markırı olan total oksidan kapasite (TOK) ve oksidatif stres indeks oranını (OSI) ise istatistiksel olarak anlamlı düzeyde azalttığını belirledik. İskemi-reperfüzyon grubu testislerde testis grupları arasında Johnsen skorlama sistemine göre istatistiksel farklılık saptanmadı. Sonuç: Bizim düşüncemize göre iloprost ve düşük doz metotreksat tedavisi, testis örneklerinde antioksidan aktiviteyi arttırmış, oksidan aktiviteyi de baskılamıştır. Ancak, histopatolojik incelemede ve spermatogenes skorlarında bir değişime neden olmamıştır.

Anahtar Kelimeler:

Testis torsiyonu, detorsiyon, antioksidan, iskemi/reperfüzyon, iloprost, düşük doz metotreksat

The Protective Effect of Iloprost and Low-Dose Methotrexate on Testicular Ischemia/Reperfusion Injury in an Experimental Model

Objective: To investigate the protective effects of iloprost and low-dose methotrexate on ischemia/reperfusion (I/R) injury in surgically detorsed testicular torsion.Material and Methods: Rats were randomly divided into 4 groups: Group 1 (sham), Group 2: Only testicular I/R; Group 3: I/R+iloprost (10 μg/kg) and Group 4: I/R+low dose metotrexate (6 mg/kg). Torsion / ischemia and detorsion / reperfusion times were determined as 4 hours. Medications were given intraperitoneally at 3 hours following onset of testicular torsion. Detorsioned right testises were removed 8 hours after the begining of the procedure in all 4 groups.Results: In our study, we found that administration of iloprost and metotrexate significantly increased total antioxidant capacity (TAC) while reducing total oxidant statüs (TOS) and oxidative stress index (OSI) levels significantly. No statistically significant difference was found for testicles in I/R groups according to the Johnsen scoring system. Conclusion: Admnistration of iloprost and low dose methotrexate treatment increased antioxidant activity in testicular samples while suppressing oxidant activity. However, there was no change in histopathological examination and spermatogenesis scores.

Keywords:

Testis torsion, detorsion, antioxidant, ischemia/reperfusion, iloprost, methotrexate,

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