Bayram KIRAN, Sana I. SOULMAN, Ergin Murat ALTUNER, Talip ÇETER, Hana Ealoma AKWIETEN, Kerim GÜNEY, Mahmut GÜR

Isırgan, Turp ve Nar Tohumlarından Soğuk-Sıkım Yöntemi ile Elde Edilen Yağların Kimyasal Bileşimi ve Antimikrobiyal Aktiviteleri

Çalışmanın amacı: Bu çalışmanın amacı ısırgan (Urtica dioica), turp (Raphanus sativus) ve nar (Punica granatum) tohumlarından elde edilen soğuk sıkım yağların antimikrobiyal etkilerinin ortaya koymaktır. Materyal ve Yöntem: Bu tohumların yağları, Staphylococcus epidermidis DSMZ 20044, Staphylococcus aureus ATCC 25923, Salmonella typhimurium SL 1344, Salmonella kentucky, Salmonella infantis, Salmonella enteritidis, Pseudomonas fluorescens P1 ATCC 13075, Pseudomonas aeruginosa DSMZ 50071, Klebsiella pneumoniae, Escherichia coli ATCC 25922, Enterococcus faecium, Enterococcus faecalis ATCC 29212, Enterobacter aerogenes ATCC 13048, Candida albicans DSMZ 1386 ve Bacillus subtilis DSMZ 1971 gibi 15 mikroorganizmaya karşı disk difüzyon metodu ve MİK testi kullanılarak, antimikrobiyal aktiviteleri açısından araştırılmıştır. Sonuçlar sefazolin, klindamisin, kloramfenikol, siprofloksasin, amoksisilin/klavulanik asit, sülfametoksazol/trimetoprim, seftriakson, gentamisin, ampisilin, sefalotin, sefuroksim ve vankomisin olmak üzere 11 standart antibiyotik ile karşılaştırılmıştır. Ekstraktlar ayrıca GC-MS kullanılarak kimyasal olarak analiz edilmiştir. Sonuçlar: Sonuç olarak, turp yağının en yüksek aktiviteyi göstererek bütün mikroorganizmalara, ısırgan yağının ise S. epidermidis hariç bütün mikroorganizmalara etkili olduğu gözlenmiştir. En düşük aktivite ise nar yağında gözlenmiştir. Önemli vurgular: Çalışmanın sonuçları, açık bir şekilde, ısırgan otu, turp ve nardan elde edilen yağların destekleyici sağlık ürünü olarak ve ilave çalışmalarla medikal amaçlı olarak da kullanılabileceğini ortaya koymaktadır.

Chemical Composition and Antimicrobial Activities of Cold-Pressed Oils Obtained From Nettle, Radish and Pomegranate Seeds

Aim of study: The aim of this study is to put forward the antimicrobial activity of cold pressed oils obtained from seeds of nettle (Urtica dioica), radish (Raphanus sativus) and pomegranate (Punica granatum).Material and Methods: Oils of these seeds were analysed for their antibacterial and antifungal activities by the disk diffusion and MIC tests against fifteen microorganisms, Staphylococcus epidermidis DSMZ 20044, Staphylococcus aureus ATCC 25923, Salmonella typhimurium SL 1344, Salmonella kentucky, Salmonella infantis, Salmonella enteritidis, Pseudomonas fluorescens P1 ATCC 13075, Pseudomonas aeruginosa DSMZ 50071, Klebsiella pneumoniae, Escherichia coli ATCC 25922, Enterococcus faecium, Enterococcus faecalis ATCC 29212, Enterobacter aerogenes ATCC 13048, Candida albicans DSMZ 1386 and Bacillus subtilis DSMZ 1971. The results were compared against 11 standard antibiotics, which are cefazolin, clindamycin, chloramphenicol, ciprofloxacin, amoxicillin/clavulanic acid, sulfamethoxazole/trimethoprim, ceftriaxone, gentamicin, ampicillin, cephalothin, cefuroxime and vancomycin. The extracts were also chemically analysed by using GC-MS.Main results: As a result, radish oil is observed to be active against all microorganisms with the highest activity, where nettle oil is active against all microorganisms except for S. epidermidis. The lowest activity was observed in pomegranate oil.Highlights: The results of the study clearly puts forward that oils obtained from nettle, radish and pomegranate could have a possible medicinal use.

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