Levamizolün Model Organizma Galleria mellonella’nın Bazı Biyolojik Özellikleri Üzerine Etkisi

İmidotiyazol türevi antihelmintik bir madde olan levamizolün büyük bal mumu güvesi, Galleria mellonella’nın, larva, pup ve ergin evresindeki yaşama oranı, gelişim süreleri ve ergin ömür uzunluğu üzerine etkileri incelendi. G. mellonella birinci evre larvaları 0,001, 0,01 ve 0,1 g/100 g levamizol içeren yapay besinler ile beslendi. Denenen levamizol konsantrasyonları levamizol içermeyen kontrol besini ile karşılaştırıldığında G. mellonella’nın yaşama ve gelişim parametrelerini üzerinde olumsuz yönde etkilediği tespit edildi. Levamizolün %0,001 g ve %0,01 g’lık konsantrasyonları kontrol grubu ile karşılaştırıldığında 7.evreye ulaşan larva oranını istatiksel olarak önemli derecede düşürdü. Denenen en yüksek konsantrasyonda (%0,1 g) bu oran kontrol grubuna göre (%98,75) 3 kat azalarak %28,75 olarak belirlendi. Levamizol içeren yapay besinlerde beslenen gruplarda kontrol grubuna göre pup olma oranı ve ergin olma oranında da benzer etkiler gözlemlendi. Levamizolün farklı konsantrasyonlarını içeren yapay besinde yetiştirilen böcekler, levamizol içermeyen kontrol grubuna göre 7.evre larva, pup ve ergin evrelere daha geç ulaştı. Levamizolün %0,001 g ve %0,01 g’ lık konsantrasyonları kontrol grubuyla karşılaştırıldığında böceğin ergin ömür uzunluğunu uzatırken denenen en yüksek levamizol konsantrasyonu (%0,1 g) böceğin ergin ömür uzunluğunu kontrol grubuna göre yaklaşık olarak 3 gün azalttı. Elde edilen sonuçlar neticesinde yapay besinle birlikte alınan levamizolün model organizma G. mellonella’nın hem aktif beslenme gösterdiği larval evrede hem de pup ve ergin dönemi olmak üzere daha sonraki gelişim evrelerinde olumsuz etkilerinin olduğu tespit edilmiştir.

Anahtar Kelimeler:

Levamizol, Galleria mellonella, yaşama oranı, gelişme süresi, ergin ömür uzunluğu

The Effect of Levamisole on Some Biological Parameters of Model Organism Galleria mellonella

The effects of levamisole, an imidothiazole derivative anthelmintic, on survival rate, development time and adult longevity of greater wax moth, Galleria mellonella, larvae, pupae and adult stage were investigated. First instar larvae of G. mellonella were fed on artificial diet containing 0,001, 0,01 and 0,1 g/100 g of levamisole. Experimented levamisole concentrations negatively affected the survival and developmental parameters of G. mellonella when compared to the control diet without levamisole. The concentrations of 0,001% g and 0,01% g of levamisole significantly decreased survival rates of 7th instar larvae when compared to the control group. Similar results obtained from the survival of pupal and adult stages. Insects that fed on artificial diet with different concentrations of levamisole reached the 7th instar larva, pup and adult stages later than the control group without levamisole. While the concentrations of 0,001% g and 0,01% g of levamisole extended adult longevity of insect, the highest concentration of levamisole tested (0,1% g) reduced the adult longevity of the insect by approximately 3 days compared to the control group. As a result, it has been demonstrated that levamisole taken with artificial diet has negative effects both in the larval stage that shows active feeding and in the subsequent developmental stages, including the pupal and adult stages of model organism G. mellonella.

Keywords:

Levamisole, Galleria mellonella, Survival rate, Developmental time, Adult longevity,

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