Effects of Oxfendazole on Metabolic Enzymes in Hemolymph of Galleria mellonella L. (Lepidoptera: Pyralidae) Larvae Reared on Artificial Diet

Oxfendazole is used as an antihelminthic drug in parasite infections of animals. Oxfendazole was added at different concentrations (0, 0.0015, 0.015, 0.15, and 1.5%) to artificial diet and the wax moth Galleria mellonella larvae were reared to 7th stage. The changes in metabolic enzymes activity in G. mellonella hemolymph tissue were investigated. It was found that the activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) was increased which are cell damage biomarkers. The highest concentration of this antihelmintic was increased the enzme activity (ALT) from 329 ± 4.33 to 529. 5 ± 3.17 U/L. Gamma glutamyl transferase (GGT) enzyme activity was increased from 0.87 ± 0.1 to 4.32 ± 0.22 U/L. Creatine kinase (CK) and amylase (AMYL) activities were significantly increased in hemolymph of G. mellonella larvae fed with the highest oxfendazole concentration (1.5%) when compared to the control group. Our results indicate that oxfendazole can be used as an insecticide with a well-adjusted concentrations because of its low acute toxicity to humans and other non-target organisms.

Oksfendazolün Yapay Besinde Yetiştirilen Galleria mellonella L. (Lepidoptera: Pyralidae) Larvalarının Hemolenf Dokusundaki Metabolik Enzimler Üzerine Etkisi

Oksfendazol, hayvanlarda görülen pazariter enfeksiyonlarda kullanılan antihelmintik bir ilaçtır. Yapay besine farklı konsantrasyonlarda (% 0, 0,0015, 0,015, 0,15, ve 1,5) oksfendazol ilave edilerek, büyük bal mumu güvesi Galleria mellonella larvaları 7. evreye kadar beslendi. G mellonella hemolenf dokusundaki metabolik enzim aktivitesindeki değişimler araştırıldı. Hücre hasarı biyobelirteci alanin aminotransferaz (ALT) ve aspartat aminotransferaz (AST) aktivitelerinin arttığı belirlendi. Antihelmintik maddenin en yüksek konsantrasyonda enzim aktivitesi 329 ± 4,33’den 529, 5 ± 3,17 U/L’ye yükseldi. Gama glutamil transferaz (GGT) enzim aktivitesi 0,87 ± 0,1’den 4,32 ± 0,22 U/L’ ye yükseldi. Kreatin kinaz (CK) ve amilaz (AMLY) enzim aktivitelerinin, en yüksek oksfendazol konsantrasyonu (% 1,5) ile beslenen G. mellonella larvalarının hemolenf doksunda kontrol grubu ile karşılaştırıldığında istatistiksel olarak önemli derecede arttığı tespit edildi. Bu çalışma; oksfendazolün insan ve diğer hedef olmayan organizmalara karşı düşük akut toksisiteye sahip olması sebebiyle konsantrasyonlarının iyi ayarlanması ile insektisit olarak kullanılabileceğini işaret etmektedir.

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