Naringeninden Elde Edilen Polimer Schiff Bazı Ligandı ve Komplekslerinin DNA Etkileşimleri

Naringenin ve etilen diaminden elde edilen 4E -4-[ 2-{[ 4E -5,7-dihidroksi-3- 4-hidroksifenil -3,4-dihidro-2H-1-benzopiran4-yliden] aminoetil imino]-2- 4-hidroksifenil -3,4-dihidro-2H-1-benzopiran-5,7-diol L1 ligandı literatüre göre sentezlenmiştir Yang and Li 2008 . Buna ek olarak Co II ve Cu II kompleksleri de monomer L1 ligandı ile elde edilmiştir. Daha sonra oksidatif poli-kondensasyon tepkimesi ile polimerleştirme gerçekleştirilerek polimerik -Schiff bazı ligandı L2 elde edilmiştir. Cu II ve Co II kompleksleri elementel analiz, manyetik duyarlık, molar iletkenlik, UV-Vis, FT-IR ve TGA/DTA ile karakterize edilmiştir. Polimer yapılı L2 ise UV-Vis., FT-IR, 1H NMR, TGA/DTA ve GPC ile yapısal olarak aydınlatılmıştır. Bizim bu çalışmadaki amacımız bu bileşiklerin DNA ile etkileşimlerini incelemektir. Bu kapsamda bileşikler direkt olarak ve H2O2 varlığında DNA ile etkileştirilmiştir. Sadece L1Cu kompleksi DNA’ya ciddi hasar vermiştir. H2O2 varlığında L2 ve L1Co bileşikleri DNA’ya hasarı belirgin bir şekilde azaltırken, L1Cu bileşiğinin DNA’ya olan hasarı artmıştır

Anahtar Kelimeler:

DNA etkileşimi, Oksidatif polikondensasyon, Polimerik Schiff bazı

DNA Interactions of Polymerized Schiff Base Ligand and Its Complexes Obtained from Naringenin

4E -4-[ 2-{[ 4E -5,7-dihydroxy-3- 4-hydroxyphenyl -3,4-dihydro-2H-1-benzopyran-4-ylidene] aminoethyl imino]-2- 4-hydroxyphenyl -3,4-dihydro-2H-1-benzopyran-5,7-diol L1 ligand derived from naringenin and ethylenediamine was synthesized according to the literature Yang and Li 2008 . In addition Co II and Cu II metal complexes were obtained with monomer ligand of L1. Then it was polymerized by oxidative polycondensation reaction to get the poly-Schiff base ligand L2 . Cu II and Co II were characterized by elemental analyses, magnetic susceptibility, molar conductivity, UV-Vis, FT-IR and TGA/DTA. L2 was characterized UV-Vis., FT-IR, 1H NMR, TGA/DTA and GPC. Our aim was to examine the interaction of these compounds with DNA. For this purpose they were used for direct interaction with DNA and presence of H2O2 experiments; serious damage to DNA only the complex of L1Cu. In the presence of H2O2, L2 and L1Co compounds were significantly reduced the damage to DNA while L1Cu increased the damage of the DNA complex.

Keywords:

DNA Interaction, poly Schiff base, oxidative polycondensation,

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