Scenedesmus sp.’nin DPPH (1,1-difenil-2-pikrilhidrazil) Serbest Radikal Süpürücü Aktivitesi

Giresun ili derelerinden izole edilen ve kültür şartları altında BG11 besi yerinde yetiştirilen Scenedesmus sp. türünün DPPH (1,1-difenil-2-pikrilhidrazil) serbest radikal süpürücü aktivite tayini için spektrofotometrik DPPH yöntemi kullanılmıştır. 10-3 M DPPH etanol çözeltisinden 1 mL alınıp 3 mL ekstrakt çözeltisi ilave edilip vorteks ile çalkalanmıştır. 30 dakika karanlıkta bekletilip 517 nm’de absorbans okunmuştur. Standart antioksidan olarak BHT ve Troloks kullanılmıştır. DPPH radikalini süpürme aktivitesi formül kullanılarak hesaplanmıştır.

Anahtar Kelimeler:

DPPH, Scenedesmus sp., alg kültür ekstraksiyonu

DPPH (1,1-diphenyl-2-picrylhydrazyl) Free Radical Sweeping Activity of Scenedesmus sp.

Spectrophotometric DPPH method was used for the determination of DPPH (1,1-diphenyl-2-picrylhydrazyl) free radical scavenging activity of Scenedesmus sp. which was isolated from the Giresun mine and cultivated under BG11 culture conditions. 1 mL of 10-3 M DPPH ethanol solution was added and 3 mL of extract solution was added and vortexed. The absorbance was read at 517 nm for 30 minutes in the dark. BHT and Trolox were used as standard antioxidants. The DPPH radical scavenging activity reaction was calculated using the formula.

Keywords:

Scenedesmus sp, DPPH, algae culture extraction,

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