Hatice Aysun MERCİMEK TAKCI, Filiz UÇAN TÜRKMEN, Pemra BAKIRHAN, Mustafa GÜNEŞ

Rheum ribes Özütlerinin Antioksidan ve Antibakteriyel Aktiviteleri

Polygonaceae familyasının türü olan Rheum ribes’in taze gövde ve yaprakları Lübnan, İran ve Türkiye’nin doğusunda sebze olarak tüketilmektedir. Son zamanlarda farmakolojik alanlarda kullanılan bu bitkiden Asya ülkelerinde saf ilaç materyalleri üretilmektedir. Çalışmamızda, Rheum ribes gövde kısımlarından etil asetat, hekzan, metanol ve saf su çözücüleri kullanılarak 10-100 mg/mL konsantrasyonlarda hazırlanan özütlerin in vitro fitokimyasal, antioksidan ve antibakteriyel aktiviteleri incelenmiştir. Çözücülere bağlı olarak ekstraksiyon verimi değişmekle birlikte en yüksek ekstraksiyon verimi (%25.3), en yüksek total fenolik (0.80±0.00 mg GAE/g) ve flavonoid içerik (0.321±0.00 mg RE/g) 100 mg/mL konsantrasyonda metanol özütünde belirlenmiştir. Rheum ribes metanol gövde özütünün DPPH· süpürme aktivitesi %91.10±0.51 olarak kaydedilmiş olup IC50 dozu 10.50 mg/mL’dir. En yüksek Fe(II) iyonlarını şelatlama aktivitesi %49.70±3.10, indirgeme kapasitesi (0.19±0.00) ve süperoksit anyonu giderme aktivitesi (%39.20±2.40) 100 mg/mL konsantrasyonda metanol özütünde belirlenmiştir. Rheum ribes’in etil asetat, hekzan ve metanol özütlerinin test bakterileri üzerinde antibakteriyel aktivite gösteremediği belirlenmiştir. Saf su özütünün gösterdiği antibakteriyel aktivite 9.00±1.00 ve 14.50±1.50 mm zon çapı aralığındadır. En yüksek inhibitör etki 14.50±1.50 mm zon çapı ile S. aureus’a karşı gözlenmiştir.

Anahtar Kelimeler:

Rheum ribes, Antioksidan, Antibakteriyel

Antibacterial and Antioxidant Activities of Rheum ribes extracts

The fresh stem and leaves Rheum ribes, species of Polygonaceae family, are consumed as vegetable in Lebanon, Iran and eastern Turkey. Recently, crude drug materials from this plant are used in pharmacological research in Asian countries. In this study, in vitro phytochemical, antioxidant and antibacterial activity of the extracts prepared at 10-100 mg/mL concentrations by using ethyl acetate, hexane, methanol and distilled water from stem parts of Rheum ribes were examined. The extraction efficiency varies depending on the solvents and the highest extraction efficiency was calculated as %25.3 at methanol extract. The highest total phenolic and flavonoid contents were determined 0.80 ± 0.00 mg GAE/g and 0.321 ± 0.00 mg RE/g at 100 mg/mL concentration of methanolic extract. DPPH· radical scavenging activity of methanolic stem extract was %91.10±0.51 (IC50 dose 10.50 mg/mL). The highest Fe(II) ions chelating activity (49.70±3.10%), reduction capacity (0.19±0.00) and superoxide anion scavenging activity (39.20 ± 2.40%) were recorded at methanol extract. Ethyl acetate, hexane and methanol extracts of Rheum ribes not showed antibacterial activity against test bacteria. Antibacterial activity of distilled water extract was in the range of 9.00±1.00 and 14.50±1.50 mm. The highest inhibitory effect was observed against S. aureus with 14.5 ±1.50 mm zone diameter.

Keywords:

Rheum ribes, Antioxidant, Antibacterial,

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