Bahar BİLGİN SÖKMEN, Yasemin SAĞKAL

Elastaz Aktivitesine Giresun Yöresindeki Bazı Yenilebilir Bitkilerin Farklı Çözücülerdeki Ekstrelerinin İnhibisyon Etkilerinin İncelenmesi

Elastazlar (E.C. 3.4.21.36), bağ dokunun önemli bir proteni olan elastini ayırma özelliğine sahip bir grup serin proteazlardır. Bu çalışmada, Giresun yöresinde yetişen 15 çeşit yenilebilir bitkinin farklı çözücülerdeki ekstrelerinin, elastaz enzim aktivitesi üzerindeki inhibitör etkisi incelendi. Bu amaçla, su, metil alkol ve etil asetat ile hazırlanan ekstrelerinin elastaz aktivitesi üzerine inhibitör etkileri spektrofotometrik olarak analiz edildi. Bu bitkiler arasında, kara lahananın (Brassica oleracea) sulu ekstresinin (IC50= 0,0002 ± 0,0001 µg/mL), söğüt otunun (Polygonum lapathifolium) metil alkollü ekstresinin (IC50= 0,0003 ± 0,0002 µg/mL) ve mendeğin (Aegopodium podagraria) etil asetatlı ekstresinin (IC50=0,0007 ± 0,0001 µg/mL) elastaz enzimine karşı en yüksek inhibitör etkisine sahip olduğu bulundu. Analizlerin sonucunda, bitki ekstrelerinin elastaz üzerinde farklı inhibitör etkilerine sahip oldukları belirlendi. Çalışmada elde edilen sonuçlar, çalışılan bitkilerin alternatif bir anti-elastaz kaynağı olarak kullanılabileceğini açıkça göstermektedir.

Anahtar Kelimeler:

Elastaz, Enzim, Enzim İnhibisyonu, İnhibitör, Bitki

Investigation of the Inhibition Effetcs of Extracts in Different Solvents of Some Edible Plants in the Region of Giresun on Elastase Activity

Elastases (Porcine pancreatic elastase, E.C. 3.4.21.36) are a group of serine proteases which possess the ability to cleave the important connective tissue protein elastin. In this study, inhibitory effects on elastase activity of the extracts in different solvents of 15 kinds of edible plants growing in Giresun province were investigated. For this purpose, inhibitory effects on elastase activity of the plants extracts which prepared with water, methyl alcohol and ethyl acetate were analysed spectrophotometrically. Among the plants , water extract of Brassica oleracea (IC50 = 0.0002 ± 0.0001 µg/mL); methyl alcohol extract of Polygonum lapathifolium (IC50 = 0.0003 ± 0.0002 µg/mL) and ethyl acetate extract of Aegopodium podagraria (IC50 = 0.0007 ± 0.0001 µg/mL) were found that they are the highest inhibitory effect against elastase enzyme. As a result of analysis, it was determined that the plant extracts showed different inhibitory effects on elastase. The data acquired in this study clearly shows that studied plants might be utilised as an alternative anti-elastase source.

Keywords:

Elastase, Enzyme, Enzyme Inhibition, Inhibitory, Plant,

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