Ahmet Burak AĞAOĞLU, Kadir GİŞİ, Murat İSPİROĞLU, Abdulkadir Yasir BAHAR, Fatma İNANÇ TOLUN, Bülent KANTARÇEKEN

Ülseratif Kolit Oluşturulan Hayvan Modelinde Arum Dioscoridis Bitki Ekstraktı Uygulamasının Antioksidan ve Anti-inflamatuvar Etkisi

Amaç Arum dioscoridis ülkemizde yetişen oksidasyon, immün sistem, inflamasyon üzerine birçok biyolojik aktiviteyi düzenlediği gösterilmiş bir bitkidir. Bizde çalışmamızda farelerde asetik asit ile oluşturulan deneysel kolitte arum dioscoridisin etkilerini araştırmayı amaçladık. Gereç ve yöntemler Ağırlıkları 180-210 gr arasında değişen 6 haftalık 56 adet Wistar Albino cinsi dişi sıçanlar her grupta 8 hayvan olacak şekilde 7 eşit gruba ayrıldı.Grup 1; kontrol grubu. Grup 2 deneysel kolit oluşturulan grup. Grup 3 profilaktik oral arum dioscoridis tedavisi verilerek deneysel kolit oluşturulan grup. Grup 4 deneysel kolit oluşturulan ve etanollü çözücüyle oral arum dioscoridis tedavisi verilen grup. Grup 5 deneysel kolit oluşturulan ve DMSO (Dimetil sülfoksit)’lu çözücüyle oral tedavi verilen grup. Grup 6 deneysel kolit oluşturulan ve DMSO’lu çözücüyle rektal tedavi verilen grup. Grup 7 deneysel kolit oluşturulan ve 2 kat oral tedavi verilen grup. Farelerden alınan doku örneklerinde arum dioscoridisin etkileri makroskopik,histopatolojik ve biyokimyasal olarak değerlendirildi. Bulgular Kolit grubunda tedavi gruplarına göre önemli kilo kaybı meydana geldi. Arum dioscoridis tedavisi ve profilaksisi ile makroskopik ve mikroskobik analizde istatistiksel olarak anlamlı iyileşme bulundu. Asetik asit ile oluşturulan kolit modelinde toplam oksidan durum, tiyol/disülfid, malondialdehit, miyeloperoksidaz seviyelerinde artış ve toplam antioksidan kapasitede azalma tespit edildi. Sonuç Bu çalışma, arum dioscoridis'in ratlarda oluşturulan kolit modelinde olumlu etkileri olduğunu ve özellikle etanol ile eritilerek verilen ve profilaksi amaçlı kullanılan gruplarda barsak iltihabını önemli ölçüde iyileştirdiğini göstermiştir.

Anahtar Kelimeler:

arum dioscoridis, oksidatif stres, deneysel kolit, total oksidan durum, malondialdehit

Intestinal Anti-Inflammatory and Anti-Oxidant Activity of The Aqueous Extract From Arum Dioscoridis in Acetic Acid Induced Colitis in Rats

Objective Arum dioscoridis is a plant that has been shown to regulate biological activity on oxidation, immune system and inflammation grown in our country. We aimed to investigate the effects of arum dioscoridis in experimental colitis related to acetic acid in rats. Material and Methods In this study 56 Wistar Albino female rats, 6 weeks old, weighing 180- 210 g, were seperated into 7 equal groups with 8 animals in each group. Group 1 is the control one without any administration. An experimental colitis has been created on Group 2. Group 3 is treated with prophylactic oral arum dioscoridis before creating experimental colitis. The rats belongs to Group 4 has been received oral arum dioscoridis treatment with ethanolic solvent after creating experimental colitis. Group 5 was given oral treatment with solvent with DMSO (Dimethyl sulfoxide) after experimental colitis was created. Group 6 rectal treatment with solvent with DMSO after experimental colitis has been created. Group 7 was given a 2-fold oral treatment after experimental colitis. In tissue samples taken from mice, the effects of arum dioscoridis were evaluated macroscopically, histopathologically and biochemically. Results In the colitis group, significant weight loss occurred compared to the treatment groups. Statistically significant improvement was found in macroscopic and microscopic analysis with arum dioscoridis treatment and prophylaxis. In the colitis model which is created with acetic acid, total oxidant status, thiol / disulfide, malondialdehyde, myeloperoxidase levels increased and a decrease in total antioxidant capacity was detected. Conclusion This study showed that arum dioscoridis has positive effects on the colitis model created in rats, and it significantly improves intestinal inflammation especially in groups given by dissolving with ethanol and used for prophylaxis purposes

Keywords:

arum dioscoridis, oxidative stress, experimental colitis, total oxidant status, malondialdehyde,

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