Mehtap NİSARİ, Dicle ÇAYAN, Erdoğan UNUR, Dilara PATAT, Ertuğrul DAĞLI, Hilal AKALIN

Triklosanın In vitro Embriyonik Rat Gelişimi Üzerine Etkisi

Triklosan, bakterilerin yağ asidi sentezini bozarak etkisini gösteren geniş spektrumlu bir antimikrobiyal ajandır. Bu çalışmanın amacı, triklosanın ratlarda in vitro embriyonik gelişim üzerindeki etkisini araştırmak ve gen ekspresyon yöntemi ile hücreleri apoptoza sürükleyen kaspaz 2, 7, 8 ve 9 değerlerini belirlemektir. Kültür şişesine eklenen triklosan dozuna göre, kontrol ve 3 deney grubu oluşturuldu. 48 saatlik kültür periyodundan sonra embriyolar gelişimsel değerlendirme amacıyla morfolojik skorlamaya tabi tutuldu. Ardından 40 embriyoya gen ekspresyonu yapılarak kaspaz-2, kaspaz-7, kaspaz-8 ve kaspaz-9 değerleri ölçüldü. Morfolojik skorlamaya ait tüm parametrelerde istatistiksel olarak anlamlı bir gerileme tespit edildi. Kaspaz-2 değerlerinin triklosan miktarı ile ters orantılı bir şekilde, gruplar arasında istatistiksel olarak anlamlı bir azalma gösterdiği, kaspaz-9 değerlerinin ise triklosan miktarıyla orantılı bir biçimde istatistiksel olarak anlamlı bir artış gösterdiği tespit edildi. Bu verilere göre, gebelik döneminde triklosan kullanımının, hücrelerde intrinsik yol aracılığıyla apoptoza yol açarak, embriyonik gelişme geriliğine neden olduğu, bu durumun doza bağlı olarak arttığı belirlendi.

The Effect of Triclosan on In vitro Embryonic Development in Rat

Triclosan (TCS) is a broad spectrum antimicrobial agent showing its effect by deactivating the fatty acid synthesis of bacteria. The aim of thisstudy was to investigate the effects of TCS on in vitro embryonic development in rats and to determine the levels of caspases 2, 7, 8, and 9inducing cells to apoptosis through gene expression. According to the TCS dose added to the culture whole rat serum, 3 experimental groupsand a control group were formed with each including 10 embryos. After 48 h culturing period, embryos were subjected to morphological scoringfor developmental evaluation. The levels of caspases 2, 7, 8, and 9 were measured by performing gene expression on 40 embryos. Significantdecreases were obtained in all parameters of morphological scoring in the experimental groups as the dose of TCS increased. While the caspase-2level showed a significant decrease among the groups and was inversely proportional to the level of TCS, the caspase-9 level showed a significantincrease among the groups and was directly proportional to the level of TCS. In conclusion, TCS was determined to cause apoptosis in the cells viathe intrinsic pathway during pregnancy period and lead to embryonic growth retardation, which increased with increased dose of TCS.

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