Canan GÜLMEZ, Kezban DALGINLI YILDIZ, Emine ATAKISI, Onur ATAKİŞİ

The Protective Effect of Lactoferrin on Adenosine Deaminase, Nitric Oxide and Liver Enzymes in Lipopolysaccharide-Induced Experimental Endotoxemia Model in Rats

In this study, the effect of lactoferrin (LF) on adenosine deaminase (ADA) activity, nitric oxide (NO) and liver enzyme levels were investigated in lipopolysaccharide (LPS)-induced experimental endotoxemia model in rats. Forty Sprague Dawley female rats were divided into four groups as control, LF (20 mg/kg, i.p.), LPS (20 μg/kg, i.p.) (E. coli type 0111:B4) and LF+LPS (20 mg/kg LF+20 μg/kg LPS, i.p.). For a week, LF was given daily, while LPS was given a single dose. Liver adenosine deaminase, nitric oxide and liver enzymes (aspartate aminotransferase ‘AST’, alanine aminotransferase ‘ALT’ and gamma glutamyltranspeptidase ‘GGT’) levels of animals sacrificed six hours after LPS injection were determined. It was found that ADA activity, considered to be a marker of cellular immunity, and NO levels increased in LPS-induced endotoxemia and LF supplementation decreased these levels significantly (P

Ratlarda Lipopolisakkarit İndüklü Deneysel Endotoksemi Modelinde Adenozin Deaminaz, Nitrik Oksit ve Karaciğer Enzimleri Üzerine Laktoferrinin Koruyucu Etkisi

Bu çalışmada, ratlarda lipopolisakkarit (LPS) ile indüklenen deneysel endotoksemi modelinde laktoferrin (LF)’in adenozin deaminaz (ADA) aktivitesi, nitrik oksit (NO) ve karaciğer enzim düzeyleri üzerine etkisi araştırılmıştır. Çalışmada 40 adet Sprague Dawley dişi rat kontrol, LF (20 mg/kg, ip), LPS (20 μg/kg, ip) (E. coli tip 0111: B4) ve LF+LPS (20 mg/kg LF+20 μg/kg LPS, ip) olmak üzere dört gruba ayrıldı. Bir hafta boyunca LF hergün verilirken, LPS tek doz uygulandı. LPS enjeksiyonundan 6 saat sonra sakrifiye edilen hayvanların karaciğer ADA aktivitesi, NO düzeyleri ve karaciğer enzim (aspartat aminotransferaz ‘AST’, alanin aminotransferaz ‘ALT’ ve gama glutamiltranspeptidaz ‘GGT’) aktiviteleri belirlendi. Hücresel immunitenin bir belirteci olan ADA aktivitesi ve NO düzeyleri LPS indüklü endotoksemide arttı ve LF takviyesi bu düzeyleri önemli şekilde azalttı (P

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