Peste des petits ruminants (PPR) virus infections in goats in the eastern Anatolia of Turkey

Bu çalışmada, klinik ve makroskobik patolojik olarak PPRV enfeksiyonundan şüphelenilen 28 farklı sürüdeki keçilerden (n=38) alınan kan, oral-nazal swap, akciğer, dalak ve lenf nodülü gibi farklı örneklerden toplam 98 materyal kullanıldı. Bu çalışmada kullanılan keçiler Doğu Anadolu Bölgesinin 11 farklı ilinde yetiştiriliyordu. PPRV enfeksiyonunun teşhisinde RT-PCR kullanıldı. RT-PCR tekniğiyle 98 materyalin 50sinde PPRV nükleik asidi belirlendi. RT-PCR sonuçlarına göre, PPRV nükleik asidi sürülerin %39.2si (11/28), örneklenen hayvanların %44.7si (17/38) ve Doğu Anadolu Bölgesinde örnekleme yapılan illerin %45.4 (5/11) inde tespit edildi. RT-PCR ile PPRV enfeksiyonunun teşhisinde oral-nazal swap ve kan gibi klinik örnekler ve lenf nodülü, dalak ve akciğer gibi nekropsi materyali teşhis değeri yüksek materyal olarak belirlendi. Bu bilgiler keçilerin PPRV enfeksiyonunun Doğu Anadoluda yaygın olduğunu ve PPRV enfeksiyonunun teşhisinde RT-PCRın duyarlı ve güvenilir bir metod olduğunu gösterdi.

Türkiyenin Doğu Anadolu Bölgesindeki keçilerde peste des petits ruminants (PPR) virus enfeksiyonları

In this study, a totally 98 materials were used consist of different samples such as blood, oro-nasal swap, lung, spleen and lymph node from goats (n=38) in the 28 flocks suspected the PPRV infection as clinically and macroscopic pathologic remarks. The goats that used in this study housed in the 11 different provinces of Eastern Anatolia. RT-PCR was used for the diagnosis of PPRV infection. PPRV nucleic acid was detected in 50 of 98 materials by RT-PCR. According to the results of RT-PCR, the PPRV nucleic acid was detected in 39.2% (11/28), 44.7% (17/38) and 45.4% (5/11) of the flocks, sampled animals and provinces in eastern anatolia, respectively. Diagnostic value of necropsy materials such as lymph node, spleen, lung and of clinical samples such as oro-nasal swap and blood were determined more valuable diagnostic materials in the diagnosis of PPRV infection by RT-PCR. Data showed that PPRV infections of the goats were widespread in the Eastern Anatolia region. Additionally, it is determined that RT-PCR is sensitive and reliable method in the diagnosis of PPRV infections.

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