Wen-Bin CHEN, Re-Jun FANG, Xin WU, Zi bin CHENG, Yun bo TIAN

The Effects of Zinc Methionine Chelate and ZnSO4 on the Growth Performance and Immune Function of the Weaned Piglets and on IPEC-J2 Cell Immune Function

Zinc Methionine chelate (Met-Zn) shows a better palatability, stability, and bioactivity than traditional zinc preparations, therefore this study evaluated the effect on the growth performance and immunologic functions of the weaned piglets. Two in vivo tests were conducted: (I) crossbreeding piglets [duroc × (landrace × large white pigs)] were fed 80 mg/kg ZnSO4 or 20, 40, 60, or 80 mg/kg Met-Zn after the weaning on day 21.The content of serum globulin and lymphocyte transformation rate were measured on days 21, 35, 45 and 60; (II) another group of piglets weaned on day 28 days were fed 80 mg/kg ZnSO4 or 80 mg/kg Met-Zn after orally administrated of Escherichia coli. The levels of some immune factors in the small intestine were measured after the feeding for one month. An in vitro experiment studied the expression of some immune factors and zinc transporters in the porcine small intestinal epithelial cells (IPEC-J2) after treatments with ZnSO4+LPS and Met-Zn+LPS. Both Met-Zn and ZnSO4 increased the lymphocyte transformation rate and the content of serum globulin. But, Met-Zn showed better effect than ZnSO4 in improving the growth performance, particularly the average daily gain, after E. coli insults. With E. coli insults, Met-Zn promoted the expression of TNF-α and IL-6 in the anterior and middle segments of the small intestine respectively, but inhibited the expression of IL-8 in the middle segment. ZnSO4 promoted the expression of IL-6 in the posterior segment of the small intestine, but inhibited the expression of TNF-α in the middle segment. Both Met-Zn and ZnSO4 dose-dependently increased the expression levels of TNF-α, IL-6, and IL-8 in IPEC-J2 cells after the LPS stimulation. In summary, Met-Zn improved the growth performance of piglets and changed the immunologic functions.

Çinko Metionin Şalat ve ZnSO4’ın Sütten Kesilmiş Domuz Yavrularında Büyüme Performansı ve Bağışıklık İle IPEC-J2 Hücre İmmun Fonksiyonları Üzerine Etkileri

Çinko metionin şalatı geleneksel çinko preprasyonları ile karşılaştırıldığında daha lezzetli, stabil ve bioaktiftir. Bu çalışmada sütten kesilmiş domuz yavrularında çinko metionin şalatın büyüme performansı ve immunolojik fonksiyonlar üzerine etkisi çalışılmıştır. İki in vivo test uygulanmıştır: (I) Melez domuz yavruları [Duroc x (Landrace x Büyük Beyaz domuz)] 80 mg/kg ZnSO4, veya 20, 40, 60 ve 80 mg/kg Met-Zn ile 21 gün süresince sütten kesme sonrasında beslendi. Serum globülin miktarı ve lenfosit transformasyon oranı 21, 35, 45 ve 60. günlerde ölçüldü. (II) 28. Günde sütten kesilmiş olan ve ağız yoluyla Escherichia coli uygulanan domuz yavruları 80 mg/kg ZnSO4 veya 80 mg/kg Met-Zn ile beslendi. Bir aylık besleme sonrasında, ince barsaklarda bazı immun faktörlerin seviyeleri ölçüldü. ZnSO4+LPS ve Met-Zn+LPS uygulaması sonrasında domuz ince barsak epitel hücrelerinde (IPEC-J2) bazı immun faktörler ve çinko transporterlerinin ekspresyonu in vitro olarak araştırıldı. Hem Met-Zn hem de ZnSO4 lenfosit transformasyon oranı ve serum globülin miktarını artırdı. Met-Zn büyüme performansını iyileştirmede, özellikle de E. coli maruziyeti sonrasında ortalama günlük kilo kazanımınd ZnSO4’tan daha iyi etki gösterdi. E. coli maruziyetinde, Met-Zn ince barsakların anterior ve orta bölümde sırasıyla TNF-α ve IL-6 ekspresyonlarını uyarırken orta bölümde IL-8 ekspresyonunu inhibe etti. ZnSO4 ince barsakların posterior bölümünde IL-6 ekspresyonlarını uyarırken orta bölümünde TNF-α ekspresyonunu inhibe etti. Hem Met-Zn hem de ZnSO4 LPS stimulasyonu sonrasında IPEC-J2 hücrelerinde TNF-α, IL-6 ve IL-8 ekspresyon seviyelerinde doza bağlı artmeya neden oldu. Özet olarak, Met-Zn domuz yavrularında büyüme performansında iyileşmeye ve immunolojik fonksiyonlarda değişime neden oldu.

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