SİNAN İNCE, YAVUZ OSMAN BİRDANE, HİDAYET YAVUZ, MEHMET ÖZDEMİR, RUHİ TÜRKMEN

The Effect of sainfoin (Onobrychis viciifolia) extract on acethylcholine, bethanechol and potassium - evoked responses on jejunum and ileum of sheep

Bu çalışmada, Onobrychis viciifolia Scop. (O. viciifolia) ekstraktının koyun jejunum ve ileum üzerindeki etkisinin ortaya konması amaçlandı. Ekstrakt (1.6 mg/ml) varlığı ve yokluğunda, asetilkolin (10-3 -10-8 M, Ak), betanekol (10-3 -10-8 M) ve potasyumun (10 - 80×10-3 M, KCl) kontraktil cevapları Emax, pD2 ve EC50 olarak belirlendi. Ayrıca ekstrakt varlığında ve yokluğunda, verapamil (10-6 veya 10-8 M) ve kalsiyumsuz Tyrode solüsyonunda Ak’nin kontraktil cevapları belirlendi. Ekstraktın kümülatif uygulaması, KCl (10-3 M) ile uyarılmış kontraksiyonları önemli derecede azalttı. Ekstrakt, Ak ve betanekolle oluşan kontraktil cevapları etkilemedi fakat KCl ile oluşan kontraktil cevapları azalttı. 1.6 mg/ml ekstrakt atropine direnç gösteren Ak ile uyarılmış kontraksiyon ve 4-diphenyl-acetoxy-N-methyl-piperidine methiodide (4-DAMP) direnç gösteren betanekol ile uyarılmış kontraksiyonları engellemedi. Kalsiyumsuz Tyrode solüsyonunda Ak’nin kontraktil cevapları azaldı ve 10-8 M verapamil buna ilave bir etki oluşturmadı. Ayrıca 1.6 mg/ml ekstrakt 10-8 M verapamil ile birlikte uygulandığında Ak’nin kontraktil cevaplarını engelledi. Çalışma sonunda ekstraktın jejunum ve ileum kas kontraksiyonlarını engellediği, bu etkisini de kalsiyumun taşınımında değişikliğe yol açarak ve kalsiyumun hücre içine girişini kısıtlayarak gösterdiği belirlendi.

Anahtar Kelimeler:

ileum, bağırsaklar, jejunum, bitki özütü, potasyum, koyun, ince bağırsak, betanekol, asetilkolin, Onobrychis viciifolia, kasılma, yem baklagilleri, engelleme

Korunga bitkisi (Onobrychis viciifolia) ekstraktının koyun jejunum ve ileumunda asetilkolin, betanekol ve potasyum ile uyarılan cevaplar üzerine etkisi

The aim of this study was to clarify the ef ect of Onobrychis viciifolia Scop. (O. viciifolia) extract on jejunum and ileum of sheep The contractile response as E , pD and EC of acetylcholine (10-3 -10-8 -3 max 2 50 M, Ach), bethanechol (10 -10-8 M) and potassium (10 - 80×10-3 M, KCl) were determined in the absence and presence of extract (1.6 mg/ml). The contractile response to Ach in the presence of verapamil (10-6 or 10-8 M) or in calcium-free Tyrode’s solution was also determined in the absence and presence of extract. Cumulative treated of extract significantly reduced the response to KCl-evoked (10-3 M) contraction. Extract did not af ect contractile response to Ach and bethanechol but decreased the contractile response to potassium. The atropine-resistant component of Ach-evoked contraction and 4-diphenyl-acetoxy-N-methyl-piperidine methiodide-resistant component of bethanechol-evoked contraction were not inhibited in the presence of 1.6 mg/ml extract. The contractile response to Ach was reduced in calcium-free Tyrode’s solution and verapamil 10-8 M had no additional ef ect. In contrast to 1.6 mg/ml extract was added together with verapamil 10-8 M, the contractile response to Ach was inhibited. In conclusion, extract inhibits jejunum and ileum muscle contractions through the inhibition of calcium inl ux and the modulation of calcium movement.

Keywords:

ileum, intestines, jejunum, plant extracts, potassium, sheep, small intestine, bethanechol, acetylcholine, Onobrychis viciifolia, contraction, fodder legumes, inhibition,

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