ZEHRA SELÇUK, Mustafa SALMAN, Nurcan ÇETİNKAYA

The Effect of Cellulase Enzyme Treatment on Digestibility of Rice Straw

Bu çalışmanın amacı, farklı düzeylerdeki selülaz enzimi muamelesi ve inkübasyonunun, çeltik samanının sindirilebilirliği üzerine olan etkisini araştırmaktır. Çeltik samanı Trichoderma reseei kaynaklı selülaz ile %0 (Kontrol, K), 1 (ÇS+SEL1), 1.5 (ÇS+SEL1.5) ve 2 (ÇS+SEL2) düzeylerinde (kuru maddede, KM'de) muamele edildi. Muamele edilen çeltik samanları, kontrol ve her bir muamele grubu için 6'şar adet cam kavanoza silolandı. Kavanozların 3 tanesi karanlıkta oda sıcaklığında (22ºC) diğer 3 tanesi de bir inkübatörde 40ºC'de 30 gün süre ile inkübasyona bırakıldı. Çeltik samanının farklı düzeylerde selülaz ile muamelesi sonucunda kontrol grubuna göre çeltik samanı in vitro gerçek sindirilebilirliğinde (IVGSyem), in vitro gerçek kuru madde sindirilebilirliğinde (IVGKMS), kuru madde bazında in vitro nötral deterjan lif sindirilebilirliğinde (IVGNDFSKM) ve in vitro organik madde sindirilebilirliğinde (IVGOMSKM) önemli artışlar (P

Sellülaz Enzimi Muamelesinin Çeltik Samanı Sindirilebilirliği Üzerine Etkisi

The aim of this study was to determine the effects of different levels of cellulase enzyme treatments and incubations on digestibility of rice straw. Rice straw was treated by using cellulase from Trichoderma reseei at 0 (Control, C), 1 (RS+CEL1), 1.5 (RS+CEL1.5) and 2% (RS+CEL2) levels (dry matter basis, DM), and treated rice straw samples were ensiled in 6 glass jars for C, RS+CEL1, RS+CEL1.5 and RS+CEL2. For control and each level of cellulase treatment, 3 glass jars were incubated at room temperature (22ºC) in the dark for 30 days, 3 glass jars were incubated at 40±0.2ºC in an incubator for 30 days. After the treatment, the significant increases (P<0.05) were determined in treatment groups compared to control for the percentage of in vitro true digestibility as fed (IVTDas fed ), in vitro true dry matter digestibility (IVTDMD), in vitro true neutral detergent fiber digestibility in DM basis (IVTNDFDDM) and in vitro true organic matter digestibility in DM basis (IVTOMDDM ) of rice straw. The best results obtained from the highest level of cellulase (RS+CEL2) treatment at 40ºC for IVTDas fed, IVTDMD, IVTNDFDDM and IVTOMDDM were 62.99±0.22, 60.43±0.23, 30.70±0.23 and 63.58±0.34%, respectively. In conclusion, observed results showed that treatment of rice straw with cellulase improved the true digestibilitiy

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