The determination of oxidative stress by paraoxonase activity, heat shock protein and lipid profile levels in cattle with theileriosis

Theileriosis, yaygın olarak görülen ve büyük ekonomik kayıplara neden olan bir hastalıktır. Bu çalışmada; theileriosisli sığırlarda, akut dönemde oksidatif stres etkisinin ısı şok protein düzeyleri, paraoksonaz aktivitesi ve lipid profili analizi (trigliserid, total kolesterol, çok düşük dansiteli lipoprotein, düşük dansiteli lipoprotein, yüksek dansiteli lipoprotein) ile ortaya konulması amaçlanmaktadır. Çalışma, 1-3 yaş arasında 15 sağlıklı ve 15 theileriosisli sığırda gerçekleştirilmiştir. Theileriosisli sığırlarda plazma malondialdehit (P

Anahtar Kelimeler:

karaciğer, Theileria, kan lipitleri, kan serumu, sığır, tespit etmek, enzim aktivitesi, tayleriyozis, triyosilgliserol

Theileriosisli sığırlarda oksidatif stresin paraoksonaz aktivitesi, ısı şok protein ve lipid profili düzeyleri ile belirlenmesi

Theileriosis is a common illness that cause large economic losses. The aim this study was to define the oxidative status by determining the changes in levels of malondialdehyde, heat shock protein, paraoxonase activity and lipid profile (triglycerides, total cholesterol, very low-density lipoprotein, low-density lipoprotein, high-density lipoprotein) in cattle with tropical theileriosis. In the study, 15 healty cattle and 15 cattle with tropical theileriosis, between 1-3 years old, were used. Plasma malondialdehyde (P<0.001) and heat shock protein 27 (P<0.01) levels were determined significantly higher in cattles with Theileriosis than the control group. On the other hand, serum paraoxonase level was significantly lower than the control group. According to lipid profile analyses, while serum trigliserid (P<0.001) and very low-density lipoprotein (P<0.01) levels were found significantly higher in cattles with theileriosis, serum total cholesterol (P<0.001), low-density lipoprotein (P<0.001) and high-density lipoprotein (P<0.001) levels were found significantly lower in cattles with theileriosis than the control group. In conclusion, serum lipid profile could play role in pathogenesis of theileriosis because of the biochemical changes, such as degeneration of liver and increased levels of lipolysis for compansate the energy requirement which was increased due to theileriosis. In addition, determination of serum paraoxonase activity and heat shock protein 27 levels may be useful tools in the determination of oxidative stress in tropical theileriosis.

Keywords:

liver, Theileria, blood lipids, blood serum, cattle, determination, enzyme activity, theileriosis, triacylglycerols,

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1. Çöl R, Uslu U: Changes in selected serum components in cattle naturally infected with Theileria annulata. Bull Vet Inst Pulawy, 51, 15-18, 2007.
2. Osman SA, Al-Gaabary MH: Clinical, haematological and therapeutic studies on tropical theileriosis in water buffaloes (Bubalus bubalis) in Egypt. Vet Parasitol, 146, 337-340, 2007.
3. Serarslan G, Altuğ E, Kontas T, Atik E, Avcı G: Caffeic acid phenetyl ester accelerates cutaneous wound healing in a rat model and decreases oxidative stress. Clin Exp Dermatol, 32, 709-715, 2007.
4. Romero FJ, Bosch-Morell F, Romero MJ: Lipid peroxidation products and antioxidants in human disease. Environ Health Persp, 106, 1229-1234, 1998.
5. Clarkson PM, Thompson HS: Antioxidants: What role do they play in physical activity and health? Am J Clin Nutr, 72, 637-646, 2000.
6. Rezaei Asri S, Dalir-Naghadeh B: Evaluation of antioxidant status and oxidative stres in cattle naturally infected with Theileria annulata. Vet Parasitol, 142, 179-186, 2006.
7. Aşkar TK, Ergün N, Turunç, V: Isı şok proteinler ve fizyolojik rolleri. Kafkas Univ Vet Fak Derg, 13 (1): 109-114, 2007.
8. Juretic D, Tadijanovic M, Rekic B, Simean-Rudolf V, Reiner E, Baricic M: Serum paraoxonase activities in hemodialyzed uremic patients. Clin Sci, 42, 146-150, 2001.
9. Aviram M, Rosenblat M, Bisgair CL: Paraoxonase inhibits high density lipoprotein (HDL) oxidation and preserves its functions: A possible peroxidative role for paraoxonase. J Clin Invest, 101, 1581-1590, 1998.
10. Dumanlı N, Aktaş M, Çetinkaya B, Çakmak A, Köroğlu E: Prevalence and distribution of tropical theileriosis in eastern Turkey. Vet Parasitol, 127, 9-15, 2005.
11. Yoshoiko T, Kawada K, Shimada T: Lipit peroxidation in maternal and cord blood and protective mechanism aganist actived-oxygen toxicity in the blood. Am J Obstet Gynecol, 135, 372-376, 1979.
12. Eckerson HW, Romson J, Wyte C: The human serum paraoxonase polymorphism: Identification of phenotypes by their response to salts. Am J Human Genet, 35 (2): 214-227, 1983.
13. Friedewald WT, Levy RI, Frederickson DS: Estimation of the concentration of low- density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem, 18, 499-502, 1972.
14. McCord JM: Human disease, free radicals and the oxidant/antioxidant balance. Clin Biochem, 26, 351-357, 1993.
15. Grewal A, Ahuja CS, Singha SPS, Chaudhary KC: Status of lipid per- oxidation, some antioxidant enzymes and erythrocytic fragility of cross-bred cattle naturally infected with Theileria annulata. Vet Res Commun, 29 (5): 387-394, 2005.
16. Ece A, Kelekçi S, Kocamaz H, Hekimoğlu A, Balık H, Yolbaş İ, Erel Ö: Antioxidant enzyme activies, lipid peroxidation, and total antioxidant status in children with Henoch-Schönlein purpura. Clin Rheumatol, 27, 163-167, 2007.
17. Yagi K: Lipid peroxides and human diseases. Chem Phys Lipids, 45 (2-4): 337-351, 1987.
18. Ciocca DR, Oesterreich S, Chamness GC, McGuire WL, Fugua SA: Biological and clinical implications of heat shock protein 27.000 (Hsp27). J Nation Cancer Instute, 85 (19): 1558-1570, 1993.
19. Ferns G, Shams S, Shafi S: Heat shock protein 27: Its potential role in vascular disease. Int J Exp Pathol, 87 (4): 253-274, 2006.
20. Ergönül S, Aşkar TK: Anaplasmosisli sığırlarda ısı şok protein (HSP), malondialdehit (MDA), nitrik oksit (no) ve interlökin (IL-6, IL-10) düzeylerinin araştırılması. Kafkas Univ Vet Fak Derg, 15 (4): 575-579, 2009.
21. Aviram M, Rosenblat M: Paraoxonases and cardiovascular diseases: Pharmacological and nutritional influences, Curr Opin Lipidol, 16, 393-399, 2005.
22. Çelik M, Gülcü F, Ozan G, Gürsu MF: Paraoksonaz 1 ve arilesteraz aktivite düzeyleri. Turk J Biochem, 30 (2): 194-199, 2005.
23. Aviram M: Does paraoxonase play a role in susceptibility to cardio- vascular disease. Mol Med Today, 5, 381-386, 1999.
24. Chiang AN, Hwang B, Shaw GC: Changes in plasma levels of lipids and lipoprotein composition in patients with Kawasaki disease. Clin Chim Acta, 260, 15-26, 1997.
25. Singh A, Singh J, Grewal AS, Brar RS: Studies on some blood para- meters of crossbred calves with experimental Theileria annulata infections. Vet Res Commun, 25, 289-300, 2001.
26. Cunha BA, Crean J, Rosenbaum G: Lipid abnormalities in babesiosis. Am J Med, 108, 758-759, 2000.
27. Aşkar TK, Salmanoğlu B, Çakmak A, Beşkaya A: Relative changes in serum lipid-lipoprotein and trace element levels in cattle babesiosis. Medycyna Weterinaria, 64 (9): 1104-1106, 2008.
28. Adamu S, Ige AA, Jatau ID, Neils JS, Useh NM, Bisalla M, Ibrahim NDG, Nok AJ, Esievo KAN: Changes in the serum profiles of lipids and cholesterol in sheep experimental model of acute African trypanosomosis. Afr J Biotechnol, 7 (12): 2090-2098, 2008.
29. Carpentier YA, Scruel O: Changes in the concentration and composition of plasma lipoproteins during the acute phase response. Curr Opin Clin Nutr, 5, 153-158, 2002.
30. Gülcü F, Gürsu FM: Paraoksanaz ve aril esteraz aktivite ölçümlerinin standardizasyonu. Turk J Biochem, 28 (2): 45-49, 2003.