Enes AKYÜZ, Mushap KURU, Mustafa MAKAV, Pınar BAYRAM

Ovariektomili Ratlarda Gentamisin İle İndüklenen Akut Böbrek Hasarına Tarantula cubensis Ekstraktının Etkisi

Bu çalışmada ovariektomili ratlarda gentamisinle indüklenen akut böbrek hasarına Tarantula cubensis ekstraktının (TCE) etkisinin araştırılması amaçlandı. Çalışmada Wistar albino, dişi, yetişkin, 40 rat kullanıldı. Randomize şekilde her grupta 8 rat olacak şekilde 5 gruba ayrıldı. Gruplar; Kontrol grubu (K), ovariektomi grubu (O), Tarantula cubensis grubu (OT), gentamisin grubu (OG), gentamisin + Tarantula cubensis grubu (OGT) olarak belirlendi. K dışındaki tüm ratlara overektomi operasyonu yapıldı. OG ve OGT’de gentamisin ile böbrek hasarı oluşturuldu. OT ve OGT’de tek doz TCE ile tedavi uygulandı. Değerlendirme için ötenazi sonrası kan, serum ve böbrek dokusu alındı. Total lökosit sayısı OT’de diğer gruplara oranla yüksek bulundu (P=0.002). Serum biyokimyasal parametrelerde, K ve O’ya kıyasla OT, OG ve OGT’de serum üre, kreatinin, aspartat aminotransferaz ve total protein seviyelerinde önemli artışlar belirlendi. OG’de serum ve böbrek dokusunda glutatyon seviyesi düşük, malondialdehit seviyesi diğer gruplara kıyasla yüksek bulundu (P

The Effect of Tarantula cubensis Extract on Gentamicin-Induced Acute Kidney Injury in Ovariectomized Rats

This study examined the effect of Tarantula cubensis extract (TCE) on gentamicin-induced acute kidney injury in ovariectomized rats. A total of 40 female Wistar albino rats were randomly divided equally into five groups: Control (C), ovariectomy (O), Tarantula cubensis (OT), gentamicin (OG), and gentamicin + Tarantula cubensis (OGT). All rats except those in the C were ovariectomized. Kidney damage was created with gentamicin for OG and OGT. The OT and OGT were treated with a single dose of TCE. Blood, sera, and kidney tissue were taken at necropsy for evaluation. Total leukocyte count was higher in the OT compared to the others (P=0.002). Significant increases were also determined in serum urea, creatinine, aspartate aminotransferase, and total protein levels in the OT, OG, and OGT compared to the C and O. The glutathione level was low in the serum and kidney tissue of the OG, and the malondialdehyde level was high compared to the others (P

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