EMEL ÜNAL TURHAN

Optimization of Entrapment Substances for Microencapsulation of Lactobacillus plantarum and Lactobacillus casei Shirota against Gastric Conditions

Microencapsulation is a promising method that has considerable effects on protection of probiotic viability. A variety of coating materials have been utilized to enhance the stability of probiotic microorganisms during the transition through gastrointestinal tract. The aim of this research was to determine optimum coating material combinations for probiotic microencapsulation against gastric conditions. Fructooligosaccharides, peptide, sodium alginate, gelatin and gellan gum were used as entrapment substances to microencapsulate Lactobacillus plantarum and Lactobacillus casei Shirota with extrusion technique. The response surface technique was applied to detect the optimum proportion of encapsulation substances against gastric condition. Microencapsulation protected probiotic cultures against stress factors such as simulated gastric juice and bile-salt solution. Optimum rate of encapsulation substances varied according to the type of probiotic bacteria. Test results showed that L. plantarum should be coated with 1.5% alginate, 0.92% gellan gum, 0.18% gelatin, 0.36% peptide and 1.31% fructooligosaccharides for highest protection. L. casei Shirota should also be coated with 2% alginate, 0.98% gellan gum, 0.51% gelatin, 0.86% peptide and 1.98% fructooligosaccharides for highest protection. This research concluded that microencapsulation with encapsulation materials at optimum concentration provided improved protection for the probiotics.

Lactobacillus plantarum ve Lactobacillus casei Shirota’nın Gastrik Koşullara Karşı Mikroenkapsülasyonu İçin Kaplama Materyallerinin Optimizasyonu

Mikroenkapsülasyon, probiyotik canlılığının korunması üzerinde önemli etkileri olan umut verici bir yöntemdir. Probiyotik mikroorganizmaların gastrik koşullara karşı dayanımını arttırmak için çeşitli kaplama materyallerinden yararlanılmıştır. Bu çalışmanın amacı gastrik koşullara karşı probiyotik mikroenkapsülasyonu için ideal kaplama materyali kombinasyonunu belirlemektir. Fruktooligosakkarit, peptit, sodyum aljinat, jelatin ve gellan gam ekstrüzyon tekniği ile Lactobacillus plantarum ve Lactobacillus casei Shirota’yı mikroenkapsüle etmek için tutuklayıcı maddeler olarak kullanılmıştır. Gastrik koşullara karşı enkapsülasyon materyallerinin ideal oranları cevap yüzey tekniği ile elde edilmiştir. Mikroenkapsülasyon işlemi yapay gastrik su ve safra tuzu çözeltisi gibi stres faktörlerine karşı probiyotik kültürleri korumuştur. Kaplama materyallerinin ideal oranları probiyotik bakteri türüne göre değişmiştir. Test sonuçları yüksek düzeyde koruma için L. plantarum’un %1.5 aljinat, %0.92 gellan gam, %0.18 jelatin, %0.36 peptit ve %1.31 FOS ile kaplanması gerektiğini göstermiştir. Yüksek düzeyde koruma için L. casei Shirota ise %2 aljinat, %0.98 gellan gam, %0.51 jelatin, %0.86 peptit ve %1.98 FOS ile kaplanmalıdır. Bu araştırma, en uygun konsantrasyonda kaplama materyalleri ile mikroenkapsülasyonun, probiyotiklerin canlılığını iyileştirdiği sonucunu çıkarmıştır.

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1. Tomar O, akarca G, Beykaya M, Çağlar a: Some characteristics of Erzincan tulum cheese produced using different probiotic cultures and packaging material. Kafkas Univ Vet Fak Derg, 24 (5): 647-654, 2018. DOI: 10.9775/kvfd.2018.19596

2. Saad N, Delattre C, Urdaci M, Schmitter JM, Bressollier P: An overview of the last advances in probiotic and prebiotic field. LWT - Food Sci Technol, 50 (1): 1-16, 2013. DOI: 10.1016/j.lwt.2012.05.014

3. Khan MI, arshad MS, anjum FM, Sameen a, Rehman a, Gill WT: Meat as a functional food with special reference to probiotic sausages. Food Res Int, 44 (10): 3125-3133, 2011. DOI: 10.1016/j.foodres.2011.07.033

4. Doğan M, Özpinar H: Investigation of probiotic features of bacteria isolated from some food products. Kafkas Univ Vet Fak Derg, 23 (4): 555562, 2017. DOI: 10.9775/kvfd.2016.17273

5. FaO/WHO: Probiotics in Food. Health and Nutritional Properties and Guidelines for Evaluation, vol. 85. FAO/WHO, Rome-Italy, 2003.

6. Klindt-Toldam S, Larsen SK, Saaby L, Olsen LR, Sivenstrub G, Mullertz a, Knochel S, Heimdal H, Nielsen DS, Zielinska D: Survival of Lactobacillus acidophilus NCFM and Bifidobacterium lactis HN019 encapsulated in chocolate during in vitro simulated passage of the upper gastrointestinal tract. LWT-Food Sci Technol, 74, 404-410, 2016. DOI: 10.1016/j.lwt.2016.07.053

7. Bernat N, Chafer M, Gonzalez-Martinez C, Rodriguez-Garcia J, Chiralt a: Optimization of at milk formulation to obtain fermented derivatives by using probiotic Lactobacillus reuteri microorganisms. Food Sci Technol Int, 21 (2): 145-157, 2014. DOI: 10.1177/1082013213518936

8. Succi M, Tremonte P, Pannella G, Tipaldi L, Cozzolino a, Coppola R, Sorrentino E: Survival of commercial probiotic strains in dark chocolate with high cocoa and phenols content during the storage and in a static in vitro digestion model. J Funct Food, 35, 60-67, 2017. DOI: 10.1016/j. jff.2017.05.019

9. Horáčková S, Žaludová K, Plocková M: Stability of selected Lactobacilli in the conditions simulating those in the gastrointestinal tract. Czech J Food Sci, 29, S30-S35, 2011. DOI: 10.17221/283/2011-CJFS

10. Makinen K, Berger B, Bel-Rhlid R, ananta E: Science and technology for the mastership of probiotic applications in food products. J Biotechnol, 162 (4): 356-365, 2012. DOI: 10.1016/j.jbiotec.2012.07.006

11. Doherty SB, Gee VL, Ross RP, Stanton C, Fitzgerald GF, Brodkorb a: Development and characterization of whey protein micro-beads as potential matrices for probiotic protection. Food Hydrocoll, 25, 1604-1617, 2011. DOI: 10.1016/j.foodhyd.2010.12.012

12. Hernandez-Hernandez O, Muthaiyan a, Moreno FJ, Montilla a, Sanz ML, Ricke SC: Effect of prebiotic carbohydrates on the growth and tolerance of Lactobacillus. Food Microbiol, 30, 355-361, 2012. DOI: 10.1016/j.fm.2011.12.022

13. Šipailienė a, Petraitytė S: Encapsulation of probiotics: Proper selection of the probiotic strain and the influence of encapsulation technology and materials on the viability of encapsulated microorganisms. Probiotics Antimicrob Proteins, 10 (1): 1-10, 2017. DOI: 10.1007/s12602017-9347-x

14. Sidira M, Kandylis P, Kanellaki M, Kourkoutas Y: Effect of immobilized Lactobacillus casei on the evolution of flavor compounds in probiotic dryfermented sausages during ripening. Meat Sci, 100, 41-51, 2015. DOI: 10.1016/j.meatsci.2014.09.011

15. Kalkan S, Öztürk D, Selimoglu BS: Determining some of the quality characteristics of probiotic yogurts manufactured by using microencapsulated Saccharomyces cerevisiae var. boulardii. Turk J Vet Anim Sci, 42, 617-623, 2018. DOI: 10.3906/vet-1804-5

16. Tee WF, Nazaruddin R, Tan YN, ayob MK: Effects of encapsulation on the viability of potential probiotic Lactobacillus plantarum exposed to high acidity condition and presence of bile salts. Food Sci Technol Int, 20 (6): 399-404, 2013. DOI: 10.1177/1082013213488775

17. Burgain J, Gaiani C, Linder M, Scher J: Encapsulation of probiotic living cells: From laboratory scale to industrial applications. J Food Eng, 104, 467-483, 2011. DOI: 10.1016/j.jfoodeng.2010.12.031

18. Li XY, Chen XG, Liu CS, Liu CG, Xue YP: Preparation of alginategelatin capsules and its properties. Front Mater Sci China, 2 (3): 253-260, 2008. DOI: 10.1007/s11706-008-0042-4

19. Chen MJ, Chen KN, Kuo YT: Optimum thermotolerance of Bifidobacterium bifidum in gellan-alginate microparticles. Biotechnol Bioeng, 98 (2): 411-419, 2007. DOI: 10.1002/bit.21450

20. Ünal E, Erginkaya Z: Probiyotik mikroorganizmaların mikroenkapsülasyonu. Gıda, 35 (4): 297-304, 2010.

21. Özer D, akin S, Özer B: Effect of inulin and lactulose on survival of Lactobacillus acidophilus La-5 and Bifidobacterium bifidum Bb-02 in acidophilus-bifidus yoghurt. Food Sci Tech Int, 11 (1): 19-24, 2005. DOI: 10.1177/1082013205051275

22. Licht TR, Ebersbach T, Frøkiær H: Prebiotics for prevention of gut infections. Trends Food Sci Techol, 23, 70-82, 2012. DOI: 10.1016/j. tifs.2011.08.011

23. Unal Turhan E, Erginkaya Z, Uney MH, Ozer Ea: Inactivation effect of probiotic biofilms on growth of Listeria monocytogenes. Kafkas Univ Vet Fak Derg, 23 (4): 541-546, 2017. DOI: 10.9775/kvfd.2016.17253

24. Unal Turhan E, Erginkaya Z, Polat S, Özer Ea: Design of probiotic dry fermented sausage (sucuk) production with microencapsulated and free cells of Lactobacillus rhamnosus. Turk J Vet Anim Sci, 41, 598-603, 2017. DOI: 10.3906/vet-1701-76

25. Chen KN, Chen MJ, Liu J, Lin CW, Chiu HY: Optimization of incorporated prebiotics as coating materials for probiotic microencapsulation. J Food Sci, 70 (5): M260-M266, 2005. DOI: 10.1111/j.13652621.2005.tb09981.x

26. Chen KN, Chen MJ, Lin CW: Optimum combination of the encapsulating materials for probiotic microcapsules and its experimental verification (R1). J Food Eng, 76, 313-320, 2006. DOI: 10.1016/j.jfoodeng. 2005.05.036

27. Harrigan WF: Laboratory Methods in Food Microbiology. 3rd edn., Academic Press Ltd., USA, 1998.

28. Lacroix C, Yildirim S: Fermentation technologies for the production of probiotics with high viability and functionality. Curr Opin Biotechnol, 18, 176-183, 2007. DOI: 10.1016/j.copbio.2007.02.002

29. Khosravi Zanjani Ma, Ghiassi Tarzi B, Sharifana a, Mohammadi N: Microencapsulation of probiotics by calcium alginate-gelatinized starch with chitosan coating and evaluation of survival in simulated human gastro-intestinal condition. Iran J Pharm Res, 13 (3): 843-852, 2014.

30. Martin MJ, Lara-Villoslada F, Ruiz Ma, Morales ME: Microencapsulation of bacteria: A review of different technologies and their impact on the probiotic effects. Innov Food Sci Emerg Technol, 27, 15-25, 2015. DOI: 10.1016/j.ifset.2014.09.010

31. Sarao LK, arora M: Probiotics, prebiotics, and microencapsulation: A review. Crit Rev Food Sci Nutr, 57 (2): 344-371, 2017. DOI: 10.1080/ 10408398.2014.887055

32. Ramos PE, Cerqueira Ma, Teixeira Ja, Vicente aa: Physiological protection of probiotic microcapsules by coatings. Crit Rev Food Sci Nutr, 58 (11): 1864-1877, 2018. DOI: 10.1080/10408398.2017.1289148

33. Nag a, Han KS, Singh H: Microencapsulation of probiotic bacteria using pH-induced gelation of sodium caseinate and gellan gum. Int Dairy J, 21, 247-253, 2011. DOI: 10.1016/j.idairyj.2010.11.002

34. González-Fernández C, Santos EM, Jaime I, Rovira J: Influence of starter cultures and sugar concentrations on biogenic amine contents in chorizo dry sausage. Food Microbiol, 20, 275-284, 2003. DOI: 10.1016/ S0740-0020(02)00157-0

35. Sathyabama S, Ranjith Kumar M, Bruntha P, Vijayabharathi R, Brindha V: Co-encapsulation of probiotics with prebiotics on alginate matrix and its effect on viability in simulated gastric environment. LWTFood Sci Technol, 57 (1): 419-425, 2014. DOI: 10.1016/j.lwt.2013.12.024

36. Shori aB: Microencapsulation improved probiotics survival during gastric transit. HAYATI J Biosci, 24, 1-5, 2017. DOI: 10.1016/j.hjb.2016. 12.008

37. Ding WK, Shah NP: Survival of free and microencapsulated probiotic bacteria in orange and apple juices. Int Food Res J, 15 (2), 219-232, 2008.

38. Ye Q, Georges N, Selomulya C: Microencapsulation of active ingredients in functional foods: From research stage to commercial food products. Trends Food Sci Techol, 78, 167-179, 2018. DOI: 10.1016/j.tifs.2018.05.025