ARMAĞAN ERDEM ÜTÜK, Fatma Çiğdem PİŞKİN, Bestami DALKILIÇ

Molecular Characterization of Sheep Isolates of Echinococcus granulosus in Kilis Province

Kistik ekinokokkozis Türkiye'de ve dünyada en önemli helmintozoonozlardan biridir. Kilis ilindeki 19 koyundan elde edilen 28 Echinococcus granulosus izolatının moleküler karakterizasyonu için mitokondrial 12S rRNA (mt-12S rRNA) ve küçük altünite ribosomal RNA (rrnS) genleri polimeraz zincir reaksiyonu (PZR) ile çoğaltıldı. Ayrıca rastgele seçilen iki koyun izolatının mitokondrial sitokrom c oksidaz altünite 1 (mt-CO1) geni kısmi olarak çoğaltılıp dizi analizi yapıldı. Çalışma sonucunda tüm koyun izolatları E. granulosus sensu stricto (G1-G3 kompleks) olarak belirlendi

Kilis İlinde Echinococcus granulosus'un Koyun İzolatlarının Moleküler Karakterizasyonu

Cystic echinococcosis is one of the most important helminthozoonosis both in Turkey and all around world. Polymerase Chain Reaction (PCR) amplification of mitochondrial 12S rRNA (mt-12S rRNA) and small subunit ribosomal RNA (rrnS) genes were used for molecular characterization of 28 isolates of Echinococcus granulosus obtained from 19 sheep in Kilis province. And also partial mitochondrial cytochrome c oxidase subunit I (mt-CO1) genes of randomly selected two sheep isolates were amplified and sequenced. At the end of the study, all sheep isolates were detected as E. granulosus sensu stricto (G1-G3 complex)

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