Aytaç AKÇAY, Fadime DALDABAN, Elif ÇELİK, Korhan ARSLAN, Bilal AKYÜZ

Meta Analysis of Allele and Genotype Frequency of Growth Hormone (bGH) Gene AluI Polymorphism, Which is Effective on Milk Yield in Holstein Cattle

There are studies investigating the relationship between bGH gene AluI polymorphism and milk yield in bovine breeds. In the presented study, the relationship between different genotypes and lactation milk yields was determined by analyzing bGH-AluI genotype and allele frequencies in Holstein breed by meta-analysis method. The study material consisted of studies investigating bGH-AluI polymorphisms that are effective on milk yield in Holstein breed. According to the determined criteria, allele and genotype frequencies of 4583 samples in 20 studies selected from 35 studies and milk yields of 932 samples in 6 studies in which milk yields were calculated according to genotypes were included in the meta analysis. In the study, meta analyzes LL, LV and VV genotypes and L and V alleles and lactation milk yields were performed for bGH gene. The study samples were proved to be unbiased with the Begg and Mazumdar Rank Correlations test and funnel plot. As a result of the study, high heterogeneity was determined between the investigated studies concerning all genotypes (LL, LV and VV) and allele frequencies (L and V), thus random effect model was used. Meta analyzes were done with Comprehensive Meta-Analysis Software (CMA). According to the random effect model, pooled ratios in LL, LV and VV genotypes are 0.710, 0.260 and 0.030; pooled ratios for L and V allele frequencies were calculated as 0.818 and 0.182 and were found statistically significant (P

Holştayn Irkı Sığırlarda Süt Verimi Üzerine Etkili Büyüme Hormonu (bGH) Geni AluI Polimorfizminin Allel ve Genotip Frekanslarının Meta Analizi

Sığır ırklarında bGH geni AluI polimorfizmi süt verim ilişkilerinin araştırıldığı çalışmalar bulunmaktadır. Sunulan çalışmada Holstayn ırkında bGHAluI genotip ve allel frekanslarının meta analizi yöntemi ile analiz edilerek, farklı genotipler ve laktasyon süt verimleri arasındaki ilişki belirlenmiştir. Çalışma materyalini, Holştayn ırkında süt verimi üzerine etkili bGH-AluI polimorfizmlerinin araştırıldığı çalışmalar oluşturmuştur. Belirlenen kriterlere göre 35 çalışma içerisinden seçilen 20 çalışmadaki toplam 4583 örneğin allel ve genotip frekansı ve seçilen çalışmalar içerisinde genotiplere göre süt verimlerin hesaplandığı 6 çalışmadaki 932 örneğin süt verimleri meta analizine dahil edilmiştir. Çalışmada bGH geni LL, LV ve VV genotipleri, L ve V allellerinin frekansları ve laktasyon süt verimleri için meta analizleri yapılmıştır. Çalışma örneklemlerinin yanlı olmadığı Begg ve Mazumdar Sıra Korelasyonları testi ve huni grafiği ile kanıtlanmıştır. Çalışma sonucunda, tüm genotiplerde (LL, LV ve VV) ve allel frekanslarında (L ve V) incelenen çalışmalar arasında yüksek heterojenite belirlenmiş, bu nedenle rassal etki modeli kullanılmıştır. Meta analizleri Comprehensive Meta-Analysis Software (CMA) ile yapılmıştır. Rassal etki modeline göre LL, LV ve VV genotiplerinde ortak frekanslar 0.710, 0.260 ve 0.030; L ve V allel frekansları için ortak frekanslar 0.818 ve 0.182 olarak hesaplanmış ve istatistiksel olarak önemli bulunmuştur (P

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