Mehmet AKAN, Ayhan BAŞTAN, Seyyide SARIÇAM İNCE, Seçkin SALAR, Ezgi DİKMEOĞLU, Tuğba OĞUZ

Keçi Mastitisinde Bakteriyel İzolasyon ve Antimikrobiyal DirençProfillerinin Tespiti

Mastitis, dünya çapında en yaygın enfeksiyonlardan biridir. Bu enfeksiyon, hayvan ve halk sağlığı açısından risk oluşturmaktadır. Bu nedenle, mastitis patojenlerinin belirlenmesi enfeksiyonun önlenmesi açısından önemlidir. Antimikrobiyal tedavi, bakteriyel mastitis tedavisinde en güvenilir ve en yaygın olanıdır. Genel olarak birden fazla antimikrobiyalin kombinasyonu, etkili bir tedavi yaklaşımıdır. Bu çalışmada, keçi sütündeki bakteriyel mastitis patojenlerinin saptanması ve bu patojenlerin antimikrobiyal direnç profillerinin belirlenmesi amaçlanmıştır. 2018-2019 yılları arasında toplam 190 keçi sütü örneği standart mikrobiyolojik analizler ile incelendi. Tüm numunelerinin %33.2’sinde bakteriyel mastitis patojenleri tespit edildi. Çoklu bakteriyel izolasyonu nedeniyle toplam 79 farklı bakteri izole edildi. Major bakteri cinsi, %44 koagülaz-negatif Staphylococcus (CNS) ve %22 koagülaz-pozitif Staphylococcus (CPS) olmak üzere Staphylococcus spp. (%66) olarak belirlendi. Staphylococcus cinsini takiben sırasıyla Bacillus spp. (%17.6), katalaz-negatif koklar (PNC) (%14), Mannheimia spp. (%1.2) ve Micrococcus spp. (%1.2) izole edildi. En yüksek prevelansa sahip türler Staphylococcus caprae (%27), Staphylococcus aureus ve Staphylococcus chromogenes (%13), Aerococcus viridans ve Bacillus cereus (%7.6) olarak tanımlandı. Antimikrobiyal direnç testi dikkate alındığında, test edilen antimikrobiyaller arasında en yüksek direnç tetrasiklinde (%31) tespit edildi. Bir Enterococcus faecalis ve üç Streptococcus uberis olmak üzere toplam 4 adet çoklu-ilaç dirençli izolat bulundu. Tüm Bacillus spp. izolatlarında en yüksek direnç oranı (%35.7) penisilin için gözlendi.

Detection of Bacterial Isolation and Antimicrobial Resistance Profiles in Goat Mastitis

Mastitis is one of the most common infections worldwide. This infection poses risks to animal and public health. Therefore, determination of mastitis pathogens is important for the prevention of the infection. Generally, the combination of antimicrobials is an effective, reliable and common treatment approach. In this study, it was aimed to determine the bacterial mastitis pathogens in goat’s milk and antimicrobial resistance profiles of these pathogens. A total of 190 goat milk samples were examined with standard microbiological analysis in the period of 2018-2019. Bacterial mastitis pathogens were obtained in 33.2% of all samples. Totally, 79 different bacterial agents were isolated due to multiple-bacterial isolation. Major genus was detected as Staphylococcus spp. (66%): coagulase-negative Staphylococcus (CNS) 44% and coagulase-positive Staphylococcus (CPS) 22%. This group was followed by respectively Bacillus spp. (17.6%), catalase-negative cocci (PNC) (14%), Mannheimia spp. (1.2%) and Micrococcus spp. (1.2%). The most prevalent species were identified Staphylococcus caprae (27%), Staphylococcus aureus and Staphylococcus chromogenes (13%), Aerococcus viridans and Bacillus cereus (7.6%). Considering the antimicrobial resistance test, tetracycline has the highest resistance rate (31%) among the tested antimicrobials. A total of 4 multi-drug resistant isolates were found: an Enterococcus faecalis and three Streptococcus uberis isolates. The highest resistance rate (35.7%) was observed for penicillin in all Bacillus spp. isolates.

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