Bayram BAYRAM, Uğur TOPALOĞLU, Asuman ARKAŞ ALKLAY, Fatma ÇELENK, Nurşin AYDIN, Ömer ATALAR

İnek Meme Bezinde Laktasyon Sonu ve İnvolusyon Döneminde Sitokeratin 8, 18 ve 19 Ekspresyonu

Sitokeratinler, epitel hücrelerinde bulunan ara filament proteinlerindendir. Ekspresyonları organ veya dokuya özgüdür ve epitel hücrelerinin tanımlanmasına izin verir, ayrıca meme kök/progenitor hücreler arasındaki ilişkiyi ortaya koyar. Bununla birlikte, geç laktasyon ve involüsyon dönemlerinde meme bezinde sitokaratinlerin ekspresyonunun karşılaştırmalı bir analizi sınırlıydı. Bu çalışmanın amacı, geç laktasyon ve involüsyon dönemlerinde ineklerde meme bezinde CK8, CK18 ve CK19 ekspresyonunu immünohistokimyasal olarak değerlendirmektir. Geç laktasyon döneminde meme bezi dokusunda lob veya lobül yapıları belirgindi, ancak bağ dokusu miktarı artmaya başlamıştı ve alveol duvarında bulunan epitel hücreleri salgı durumuna göre farklı görünümlere sahipti. Geç laktasyon süresince kanalların duvarları tek katlı kolumnar epitel hücreleri ile örtülmüştü. CK8 ve CK18, geç laktasyon ve involusyon dönemlerinde lob ve lobüllerin içindeki kalın epitel hüclerinde güçlü ekspresyonlar göstermiştir. Hem geç laktason döneminde hem de involusyon dönemlerinde meme bezi dokusunda CK 19 ekpresyonu yoktu. Sonuç olaral geç laktasyon ve involusyon döneminde ineklerde meme bezinin alveolar ve duktal epitel hücrelerinde CK8 ve CK18’in eksprese edildiği, ancak CK19’un eksprese edilmediği gösterildi. Böylece, çalışma bulgularımız CK8 ve CK18’in meme epitel farklılaşması ve normal meme epitel tabakasının korunmasındaki rolünü ortaya koydu.

Expression of Cytokeratin 8, 18 and 19 in the Period of Late Lactation and Involution in Cow Mammary Gland

Cytokeratins are intermediate filament proteins found in epithelial cells. Specific cytokeratin expression has been suggested to mark diff erent epithelial cell line and also to associate with mammary stem/progenitor cells. However, a comparative analysis of the expression of cytokaratins in the mammary gland during the late lactation and involution periods was limited. Here, the aim of the present study is to evaluate CK8, CK18 and CK19 expressions of the mammary gland in cows during late lactation and involution periods, immunohistochemically. Th e lobe and lobule structure were prominent in the mammary tissue in the late lactation period, but the amount of connective tissue started to increase, and epithelial cells found on the walls of the alveoli had diff erent appearances based on their secretion status. Th e walls of the ducts were covered by simple columnar epithelial cells during late lactation. CK8 and CK18 showed strong expressions in the epithelial cells of several alveoli and ducts inside the lobes and lobules in the late lactation and involution periods. In both late lactation and involution periods, there was no CK19 expression in the mammary gland tissue. In conclusion, it was demonstrated that CK8 and CK18 were expressed in the alveolar and ductal epithelial cells of the mammary gland in cows in the late lactation and involution periods, but CK19 was not expressed. Th us, our study findings revealed the role of CK8 and CK18 in mammary epithelial diff erentiation and maintenance of the normal mammary epithelial layer.

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