İclal SARAÇOĞLU, Vahap Murat KUTLUAY, Zeynep DOĞAN, Yasir IHTESHAM, Uzma KHAN

Incarvillea emodi (Royle ex Lindl.) Chatterjee’nin Bazı Biyolojik Etkilerinin Değerlendirilmesi ve Aktif Bileşiklerinin Tayini

Bu çalışmada, Incarvillea emodi’nin antioksidan ve sitotoksik etkileri araştırılmıştır. Pakistan’ın farklı bölgelerinden toplanan Incarvillea emodi’nin farklı kısımlarından hazırlanan sulu ekstrelerin radikal süpürücü etkileri, 2,2-difenil-1-pikrilhidrazil (DPPH), nitrik oksit (NO), süperoksit (SO) ve 2,2’-azino-bis 3-etilbenzotiyazolin-6-sülfonik asit (ABTS•+) radikallerine karşı incelenmiştir. Bir ekstrenin üç poliamit kolon fraksiyonunun DPPH radikal süpürücü etkileri de araştırılmıştır. Ekstrelerin sitotoksik aktiviteleri, MTT [3-(4,5-dimetiltiyazol-2-il)- 2,5-difeniltetrazolyum bromür] yöntemi kullanılarak Hep-2 (insan larinks epidermoit karsinoma) kanser hücre dizisine karşı saptanmıştır. Ayrıca, en etkili poliamit kolon fraksiyonunda, feniletanoit glikozitlerinin varlığını göstermek için bir HPLC-DAD sistemi geliştirilmiştir. Ekstrelerin serbest radikal süpürücü etkileri, referans antioksidanlar, 3-t-butil-4-hidroksianizol (BHA), kersetin ve askorbik asit (AA) ile karşılaştırılabilir seviyede bulunmuştur. Hep-2 kanser hücre dizisine karşı konsantrasyona bağlı sitotoksik aktivite gözlenmiştir. HPLCDAD sistemine uygulanmış etkili poliamit kolon fraksiyonunda, akteozit ve lökoseptozit A isimli 2 feniletanoit glikozitinin bulunduğu tespit edilmiştir. Incarvillea emodi’de feniletanoit glikozitlerinin varlığı ilk kez bu çalışma ile gösterilmiştir. Antioksidan ve sitotoksik aktivite araştırmaları ilaç geliştirme çalışmalarında önemlidir. Çalışmamızın sonuçları, Incarvillea emodi’nin, Pakistan’da çeşitli biyolojik etkileri nedeniyle halk arasında kullanılışını desteklemektedir. Ayrıca, Incarvillea cinsinin dispepsi ve iç hastalıklarda geleneksel etnoveteriner ilaç olarak kullanımı da daha önceki çalışmalarda belirtilmektedir.

Evaluation of Some Biological Effects of Incarvillea emodi (Royle ex Lindl.) Chatterjee and Determination of Its Active Constituents

This present study was an evaluation of the antioxidant and cytotoxic activities of Incarvillea emodi (Bignoniaceae). The aqueousextracts of different parts of Incarvillea emodi, collected from different places in Pakistan, were tested for radical scavenging effectson 2,2-diphenyl-1-picrylhydrazil (DPPH), nitric oxide (NO), superoxide (SO) and [2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)](ABTS•+) radicals. DPPH radical scavenging effects of three polyamide column fractions of one extract were also tested. The cytotoxicityof the extracts were tested against Hep-2 (human larynx epidermoid carcinoma) cancer cell line by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] method. In addition, an HPLC-DAD system was used to show the presence of phenylethanoid glycosidesin the most active polyamide column fraction. The free radical scavenging effects of the extracts were found comparable to that ofreference antioxidants, 3-t-butyl-4-hydroxyanizole (BHA), quercetin and ascorbic acid (AA). Concentration dependent cytotoxic activitywas observed against Hep-2 cancer cell line. Two phenylethanoid glycosides, acteoside and leucosceptoside A, were identified in theactive polyamide column fraction at HPLC-DAD system. The presence of phenylethanoid glycosides in Incarvillea emodi was shownfor the first time with this study. Antioxidant and cytotoxic activity researches are important for developing new drugs. Our resultssupported to use of Incarvillea emodi as folk medicine due to several biological effects in Pakistan. Moreover, the use of Incarvillea genusas a traditional ethnoveterinary medicine in dyspepsia and internal diseases were reported in previous studies.

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