Atila Taner KALAYCIOĞLU, Rıza DURMAZ, Dilek GÜLDEMİR, Gülay KORUKLUOĞLU, Mustafa ERTEK

Genetic analysis of the partial M RNA segment of crimean-congo hemorrhagic fever viruses in Turkey

Kırım-Kongo kanamalı ateşi (KKKA), kene kaynaklı, ölümcül bir zoonotik hastalık olup, Doğu Avrupa, Asya, Afrika ve Balkan yarımadasında yaygın olarak görülmektedir. Türkiyede 2002 yılından itibaren artan bir sıklıkta görülmektedir. KKKAVleri genetik analizi, yakın zamanda yapılan iki çalışmada 2006 ile 2010 yıllarını kapsayan süreçte araştırılmıştır. Kırım Kongo hastalığının ülkemizde önemli sağlık sorunu oluşturması, gelecek için hastalık sürecinin değerlendirilmesi ihtiyacı; virüsün genetik yapısının sürekli izlenmesini gerekli kılmaktadır. Bu çalışmada 2011 ve 2012 yılları arasındaki iki yıllık süreçte hastalardan elde edilen KKKA virüs (KKKAV) izolatlarının genetik analizi amaçlanmıştır. KKKAV pozitif 10 hastanın serumlarından elde edilen RNA örnekleri kullanılarak kısmı M segment sekansları elde edilmiştir. Elde edilen sekansların eşleştirilmesi sonucu izolatların sekans benzerliği %94.4 ile %100 arasında bulunmuştur. Filogenetik analiz sonucu sekans analizi yapılan izolatların Avrupa I kümesinde yer aldığı, Türkiye, Doğu Avrupa-Rusya ve Balkan Yarımadasında daha önce analiz edilen virüslerle yakın ilişkili olduğu tesbit edilmiştir. Bu çalışmada Türkiyede dolaşımda olan KKKAVlerinin genetik olarak sabit ve çeşitlilik yönünden de kısıtlı olduğu sonucu gözlenmiştir.

Türkiyedeki kırm kongo kanamalı ateşi virüslerinin kısmi M-segmentlerinin genetik analizi

Crimean-Congo hemorrhagic fever (CCHF) is a fatal tick-borne zoonosis extensively common in Africa, Asia, Eastern Europe and the Balkan Peninsula. CCHF has been reported in Turkey with high frequency since 2002. Genetic diversity of CCHF virus (CCHFV) isolates circulating in Turkey were studied by two recent studies from 2006 to the end of 2010. Since CCHFV disease has been an important public health concern in Turkey, it is necessary to continue genetic analysis of CCHFV viruses for the assessment of future patterns of disease. The aim of the present study was to genetic analysis of CCHFV isolates derived from infected patients over a two-year period (2011 and 2012) in several provinces of Turkey. Serum samples (n=10) were selected from CCHFV RNA positive patients and subjected to sequence analysis of the gene region encoding partial M segment. The nucleotide sequence alignments of the 10 partial M segments of CCHFV isolates showed that the nucleic acid relatedness of CCHFV isolates ranged from 94.4% to 100%. Phylogenetic analysis of M segment sequences revealed that CCHFV isolates circulating in Turkey belonged to the European lineage I and were closely related to the viruses previously found in Turkey and in the Eastern European-Russian and Balkan Peninsula. The results of the present study indicated the genetic stability and the lack of the genetic diversity of CCHFV isolates circulating in Turkey.

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