Jingjing REN, Mingwei YANG, Pengyan WANG, Jianjun JIANG, Genqiang YAN

Farelerde Düşük Virulans Gösteren ve Listeriosise Karşı Koruma Sağlayan Listeria monocytogenes Mutantı

Bu araştırma güvenli ve yüksek derecede immunojenik bir Listeria suşu elde etmek ve delesyon mutantının biyolojik özelliklerini değerlendirmek için yapıldı. Homolog rekombinasyon teknolojisi kullanılarak, L. monocytogenes’in bir delesyon mutantı olan Lm90-ΔinlB oluşturuldu. Aynı zamanda, farelerde listeriosis enfeksiyonuna karşı güvenliği ve koruyucu etkinliği belirlendi. Sonuçlar, Lm90-ΔinlB’in virülansının, parental suşa (Lm90) kıyasla önemli ölçüde azalabileceğini gösterdi. Delesyon mutantı suş hemolitik aktiviteyi korudu ve CD8+ T hücre tepkisini Lm90 ile karşılaştırılabilir düzeyde uyardı. Lm90- ΔinlB ile immünize aşılanmış farelerde, Lm90’a eşdeğer seviyelerde LLO91-99 ve P60217-225 listeria epitoplarına spesifik CD8+ T hücrelerini uyarabilme kapasitesi mevcuttu. Farelerde Lm90-ΔinlB ile aşılanması sonrası listeriyoza karşı iyi bir koruma görülmesi önemli bir bulgudur. Sonuç olarak, Lm90-ΔinlB suşu, parental suşundan daha fazla immünojenik ancak önemli ölçüde daha az toksik olması nedeniyle potansiyel bir aşı adayıdır.

A Mutant of Listeria monocytogenes Shows Decreased Virulence and Confers Protection Against Listeriosis in Mice

This research was performed to obtain a safe and highly immunogenic Listeria strain and evaluate the biological characteristics of the deletionmutant. Based on homologous recombination technology, we constructed a deletion mutant Lm90-ΔinlB of L. monocytogenes. Meanwhile, wecharacterized its safety and protective efficacy against listeriosis infection in mice. The results showed that the virulence of Lm90-ΔinlB couldsignificantly decrease compared with the parental strain (Lm90). The deletion strain retained hemolytic activity and induced CD8+ T cell responsecomparable to that of Lm90. Mice immunized with Lm90-ΔinlB were capable of stimulating specific CD8+ T cells to the listerial epitopes LLO91-99and P60217-225 at levels equivalent to Lm90. Importantly, immunization of mice with Lm90-ΔinlB displayed good protection against listeriosis. Inconclusion, strain Lm90-ΔinlB is a vaccine candidate with the potential to be more immunogenic yet considerably less toxic than the parental strain.

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