Excretion of coxiella burnetii in cows with secretion disorder
Coxiella burnetii zoonotik potansiyele sahip çok önemli bir hastalığa neden olan bir ajandır. Etkeni insanlara aktarmada evcil ruminantlar oldukça önemlidir. Etken enfekte hayvanların dışkı, idrar, vajinal akıntılar, plasenta ve sütlerinde bulunabilir. Tüm bu yollar arasında süt ile salınım en uzun süren salınım yolu olup birkaç laktasyon periyodu süresince devam edebilir. Bu makalede, seropozitif ineklerde süt yoluyla Coxiella burnetii atılımı ile süt ve kan serumunda immunglobulin G seviyelerinin dinamikleri incelendi. Ayrıca bu parametreler arasındaki ilişki de değerlendirildi. Enfekte ineklerden elde edilen süt örneklerinde somatik hücre sayımı yapıldı. Coxiella burnetii genomunu belirlemek amacıyla PCR metodu uygulandı. Immünoglobulin konsantrasyonunu belirlemek için RID plaklar kullanıldı. Patojenin süt ile salınımı laktasyon devreleri arasında farklılık gösterdi; birinci evrede en düşük olup (%80.0) ikinci evrede en yüksek seviyedeydi (%16.6). Süt serumunda immunglobulin G konsantrasyonu en düşük ikinci evrede belirlendi (4.0 g). Somatik hücre sayısı artmış olup bazı örneklerde çok yüksekti.
Salgı bozukluğu olan ineklerde coxiella burnetiinin atılımı
Coxiella burnetii is the causative agent of a very important disease with zoonotic potential. Domestic ruminants are very important factor in transmission of this pathogen to humans. This pathogen can be found in feces, urine, lochia, placenta and milk of infected animals. Of all these routes, the excretion through milk lasts the longest, and can last over several lactations. In this paper, we examined the excretion dynamics of Coxiella burnetii through milk of seropositive cows and the levels of immunoglobulin G in milk and blood serum. Correlation between these parameters is also examined. Milk samples from infected cows were used for somatic cell count determination. The PCR method was used for determination of Coxiella burnetii genome. RID plates were used for determination of concentration of immunoglobulin. The excretion of the pathogen through milk difered between diferent stages of lactation: it was the lowest in the first stage (16.6%) and the highest in the second stage (80.0%). In the second stage the lowest concentration of immunoglobulin G was detected in milk serum (4.0 g). Somatic cell count was increased, some samples had very high values.
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