CEVDET UĞUZ, ÖMER VARIŞLI, Cansu AĞCA, Yüksel AĞCA

Effects of nonylphenol on motion kinetics, acrosome and mitochondrial membrane potential in frozen-thawed bull sperm

Son çalışmalar çevre toksini olarak bilinen Nonylpfenol (NP)’in, değişik türlerin sperm parametreleri üzerine zararlı etkisinin olduğunu göstermiştir. Bu çalışma NP’in dondurulmuş-çözdürülmüş boğa sperması üzerine etkisinin tespit edilmesi amacı ile yapılmıştır. Sperma örnekleri Ethanol veya DMSO içerinde değişik yoğunlukta (1, 10, 100, 250 ve 500 μg/ml) çözdürülen NP ile 4 saat 37oC’de inkübe edildi. Sperm parametreleri inkübasyonun 0., 1., 2., 3. ve 4. saatlerinde ölçüldü. Hem motilite ve hem de mitokondrial membran potansiyeli 250 μg NP/ml ≥ dozlara sahip gruplarda önemli derecede düşmüştür (P

Nonylphenol‘ün dondurulmuş-çözdürülmüş boğa sperma hareket kinetiğine, akrozom ve mitokondrial membran potansiyeline etkisi

Recent reports have shown that the Nonylphenol (NP), a well-known toxic environmental chemical has adverse effects on sperm in different species. Therefore, the current study was aimed to determine the deleterious effect of NP on frozen-thawed bull sperm. Sperm were exposed to different concentrations of NP (1, 10, 100, 250 and 500 μg NP/mL) dissolved either in DMSO or Ethanol and incubated for 4 h at 37oC. Sperm parameters were assessed at 0, 1st, 2nd, 3rd, and 4th h of incubation. Both percent motility and mitochondrial membrane potential (MMP) of sperm have decreased significantly (P<0.05) at dose level of ≥ 250 μg NP/mL. Acrosome reaction was induced (P<0.05) even at minimal dose concentrations of 1μg NP/mL (P<0.05). However, the percentage of acrosome reacted sperm increased (P<0.05) with increase in dose level. In conclusion, NP impairs the sperm characteristics by adversely affecting motion kinetics, mitochondrial membrane potential and inducing premature acrosome reaction in bovine.

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