Mehmet TUZCU, EMRAH SUR, İLHAMİ ÇELİK, YASEMİN ÖZNURLU, MUSTAFA KEMAL ÇİFTÇİ

Effects of aflatoxin on the proportions of peripheral blood leukocytes and alpha-naphtyl acetate esterase (ANAE) positive lymphocytes in the mouse

Bu çalışmada aflatoksinin (AF) farelerin lökosit formülü ve perifer kan lenfositlerinin alfa naftil asetat esteraz (ANAE) pozitifliği üzerindeki etkileri araştırılmıştır. Çalışmada 40 adet beyaz fare 8 fareden oluşan 5 gruba ayrıldı ve birinci grup kontrol grubu olarak standart diyetle, diğer 4 grup ise sırasıyla 200 ppb, 400 ppb, 800 ppb ve 1600 ppb AF içeren diyetle beslendi. Sonuç olarak aflatoksin içeren diyetle beslenen gruplarda doza bağlı olarak perifer kan lenfosit oranları ile ANAE pozitif lenfosit (T-lenfosit) oranlarında düşüşler ortaya çıktı. Bu sonuçlar hücresel immunite üzerinde aflatoksinlerin baskılayıcı etkisinin olabileceğini hematolojik olarak ortaya koymuştur.

Anahtar Kelimeler:

aflatoksin zehirlenmesi, aflatoksin, hayvan modelleri, hücresel bağışıklık, diyet, hematoloji, akyuvarlar, lenfositler, fareler, mikotoksikoz, zehirlenme, toksisite

Farelerde aflatoksinin perifer kan lökositleri ve alfa-naftil asetat esteraz (ANAE) pozitif lenfositlerin oranlarına etkisi

In this study detrimental effects of aflatoxin (AF) on the peripheral blood formula and the alpha naphthyl acetate esterase (ANAE) - positivity profile of peripheral blood lymphocytes (PBL) on the mice were determined. A total of 40 white mice were randomly divided in to 5 groups each having 8 animals. The first group served as a control and received standard diet. The remaining 4 groups were fed diets containing 200, 400, 800 and 1600 ppb AF, respectively. The results of this study have revealed that AF given by the food caused significant declines both in the proportion of PBL and in the percentages of ANAE-positive peripheral blood lymphocytes (T-lymphocytes) in a dose dependent manner. These results may be concerned as a haematological evidence for immuno-suppressive effect of AF on cell-mediated immunity.

Keywords:

aflatoxicosis, aflatoxins, animal models, cell mediated immunity, diet, haematology, leukocytes, lymphocytes, mice, mycotoxicoses, poisoning, toxicity,

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