Sema İşisağ ÜÇÜNCÜ, ÖZLEM ÖNEN ÇELEBİ, Gürsel ERGEN, Melih ÜRETEN, Emrah BOZ, Kenan SEFEROĞLU, Burak GÖKÇE

Dioktil adipat’ın (DOA) Labidochromis caeruleus’un fryer, 1956 (Cichlidae, teleostei) solungaç histolojisi üzerine etkileri

Dioktil adipat (DOA), ikincil plastikleştirici olarak başta polivinil ve diğer polimerler olmak üzere birçok maddenin üretiminde kullanıldığı halde ekotoksikolojik etkileri hakkında çok az bilgi vardır. Subletal düzeyde ve kısa süreli DOA maruziyetinin Labidochromis caeruleus’un (sarı prenses) solungaçları üzerindeki birincil etkilerinin belirlenmesi amacıyla balık örnekleri iki kontrol ve bir deneme grubu olmak üzere üç gruba ayrılmıştır. 30 günlük deneme süresi sonunda solungaç dokuları histolojik olarak incelenmiştir. 0.75 ppm DOA uygulanan deneme grubundan alınan doku örnekleri, hiçbir uygulama yapılmayan ve 2.5 ppm aseton uygulanan kontrol grupları ile karşılaştırıldığında; hipertofi, ciddi ölçüde hiperplazi, anevrizma, ödem ve çarpıcı füzyon olarak ayırt edilen histopatolojik değişimler gösterdikleri izlenmiştir. Sonuçlar, DOA’nın bir çevre kirleticisi olarak daha fazla dikkate alınması gerektiğini işaret etmektedir.

Anahtar Kelimeler:

klinik görünüm, balık, solungaçlar, histopatoloji, morfoloji, subletal etkiler, toksikoloji

The effects of dioctyl adipate (DOA) on the gill histology of Labidochromis caeruleus fryer, 1956 (Cichlidae, teleostei)

Although it is used widely as a second plasticizer in processing polyvinyl and other polymers, the data about the ecotoxicological effects of dioctyl adipate (DOA) is limited. In order to investigate the primer effects of short term sublethal exposure to DOA on the gills of Labidochromis caeruleus (yellow princess), the specimens were divided into three groups included two control and one experimental group. After a period of 30-days, the tissues were removed for histological examination. When compared to the untreated and 2.5 ppm acetone exposed controls, the gills of the experimental animals that exposed to 0.75 ppm DOA showed remarkable histopathological changes distinguished as hypertropy, severe hyperplasia, aneurysma, oedema, epithelial lifting, and striking fusion. The results indicate that DOA would have to take into more consideration as an ecotoxicant.

Keywords:

clinical aspects, fishes, gills, histopathology, morphology, sublethal effects, toxicology,

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