YAHYA KUYUCUOĞLU, ESRA ŞEKER, BARIŞ SAREYYÜPOĞLU, ZEKİ GÜRLER

Detection of enterohemolysin and intimin genes in Escherichia coli O157:H7 strains isolated from calves and cattle in Afyonkarahisar - Turkey

Bu çalışmada, 457 dışkı örneğinden (237 buzağı ve 220 sığır) izole edilen 14 Escherichia coli O157:H7 suşunun enterohemolizin (EhlyA) ve intimin (eaeA) virulens genlerinin PZR ile belirlenmesi amaçlandı. On üç suşta (%92.8) EhlyA geni saptanırken, 8 suşta (%57.1) eaeA geni pozitifti. Sekiz eaeA geninin 4’ü (%50.0) ishalli, 2’si (%25.0) ishalsiz buzağılar ve 2’si de (%25.0) klinik olarak sağlıklı görünen sığırlarda belirlendi. Toplam 7 (%50.0) suşun hem EhlyA hem de eaeA geni taşıdığı belirlendi. Bu çalışma ile özellikle ishalli buzağıların ve sığırların, insanlar için patojenik olabilecek E. coli O157:H7 rezervuarı oldukları doğrulandı.

Anahtar Kelimeler:

Afyon, soy, halk sağlığı, izolasyon, genler, sığır, buzağı, Türkiye, Escherichia

Afyonkarahisar’da sığır ve buzağılardan izole edilen Escherichia coli O157:H7 suşlarında intimin ve enterohemolizin Genlerinin belirlenmesi

The aim of this study was to detect enterohemolysin (EhlyA) and intimin (eaeA) virulence genes of 14 Escherichia coli O157:H7 strains isolated from 457 fecal samples (237 calves and 220 cattle) by PCR. While EhlyA gene was determined in 13 (92.8%) strains, the eaeA gene was positive in 8 (57.1%) strains. Of the 8 eaeA genes, 4 (50.0%) were obtained from diarrheic calves, 2 (25.0%) from non-diarrheic calves, and 2 (25.0%) from healthy cattle. A total of 7 (50.0%) strains were determined to harbour both of the EhlyA and eaeA genes. This study confirmed that calves, especially diarrheic, and cattle are a reservoir of E. coli O157:H7 strains that may be pathogenic for human.

Keywords:

Afyon, strains, public health, isolation, genes, cattle, calves, Turkey, Escherichia,

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