Alireza LOTFI, Habib Aghdam SHAHRYAR, Mohammad NARIMANI RAD, Jalil Dolgharı SHARAF

Changes in the differential leukocyte count in newly hatched chicks following In ovo ghrelin administration

Bu çalışmanın amacı in ovo ghrelin enjeksiyonunun yeni çıkan civcivlerin lokosit sayısı ve heterofil/lenfosit (H/L) oranı üzerine etkilerinin incelemesidir. Toplam 250 embriyolu yumurta 5 guruba ayrıldı; kontrol veya grup 1 (enjeksiyon uygulanmayan), grup 2 (5. günde in ovo 50 ng ghrelin enjekte edilen), grup 3 (5. günde in ovo 100 ng ghrelin enjekte edilen), grup 4 (10. günde in ovo 50 ng ghrelin enjekte edilen) ve grup 5 (10. günde in ovo 100 ng ghrelin enjekte edilen). Kuluçka çıkım sonrası kan örneklerinde lenfosit, heterofil, monosit, eozinofil ve bazofil sayıları ile H/L oranı belirlendi. Lökosit tipleri sayıları (lökosit formülü) kan frotisinin boyanmasından sonra 100 beyaz kan hücresi gözlemlenerek gerçekleştirildi. Lökosit sayısı kontrol grubunda (%62) enjeksiyon uygulanan grup 2, 3, 4 ve 5 ile (sırası %60, 56, 58 ve 53.6 oranlarında) kıyaslandığında yüksek bulundu. Onuncu günde yüksek dozda (100 ng) in ovo ghrelin enjeksiyonu, kontrol grupla kıyaslandığında heterofil, monosit, eozinofil ve bazofil sayılarının artmasına neden oldu. Sonuç olarak, inkubasyon sırasıda özellikle 10. günde ve 100 ng dozda in ovo ghrelin uygulaması, heterofil, bazofil ve eozinofil sayılarını ve H/L oranını arttırarak ve lenfosit sayısını azaltarak lökosit formülünü değiştirmektedir.

Anahtar Kelimeler:

yumurta, civciv, bazofil, kümes hayvanları, monosit, lenfositler, akyuvar sayısı, yumurtadan çıkmak, eozinofil

In ovo ghrelin enjeksiyonunun yeni çıkan civcivlerin Lökosit sayıları üzerine etkisi

The aim of this study was to investigation on effects of in ovo administration of ghrelin on leukocytes count and heterophil/lymphocyte (H/L) ratio in hatched chicks. 250 fertile eggs were divided to 5 groups; control or group 1 (without injection), group 2 (in ovo injected with 50 ng ghrelin at day 5), group 3 (in ovo injected with 100 ng ghrelin at day 5), group 4 (in ovo injected with 50 ng ghrelin at day 10) and group 5 (in ovo injected with 100 ng ghrelin at day 10). After hatching and blood sampling, lymphocyte, heterophil, monocyte, eosinophil and basophil count and H/L ratio were determined. The differential count of leukocytes was done observing 100 white blood cells, after staining the glass slides containing the spread blood. It was observed that lymphocyte count was more in control group (62%) in comparison with injected groups 2, 3, 4 or 5: 60, 56, 58 and 53.6% respectively). In ovo ghrelin injection at day-10 caused increases heterophil, monocyte, and eosinophil and basophil number in comparison with control group, that higher dosage (100 ng) caused significant elevation. It was concluded that in ovo ghrelin administration during incubation especially at day-10 and in 100 ng dosage can changes differential leukocyte count with increase heterophil, basophil and eosinophil counts and H/L ratio and decreases lymphocytes.

Keywords:

eggs, chicks, basophils, poultry, monocytes, lymphocytes, leukocyte count, hatching, eosinophils,

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