Antibiotic Resistance Gene Profiles of Staphylococcus aureus Isolated From Foods of Animal Origin

Bu çalışmada hayvansal gıdalardan izole edilen Staphylococcus aureus izolatlarının antibiyotik dirençlilik ve ilgili gen profillerinin araştırılması amaçlanmıştır. Besin Hijyeni ve Teknolojisi laboravuarımızda yer alan kültür koleksiyonunda, 2009 ve 2012 yılları arasında toplanan 95 S. aureus izolatları, fenotipik testler ve PCR ile doğrulanmıştır. İzolatların antibiyotiklere duyarlılıkları disk difüzyon testi ile, minimal inhibitor konsantrasyonları ise E test ile incelenmiştir. Ayrıca çalışmada PCR, blaZ, ermA, ermC, tetK, tetM, mecA, VanA, VanB, VatA, VatB ve aacA-aphD genlerinin varlığını tespit etmek amacıyla kullanılmıştır. Testler sonunda penisilin, tetrasiklin, vankomisin, eritromisin, sefoksitin, gentamisin ve quinupristin-dalfopristin antibiyotiklerine karşı direnç oranları sırasıyla %81.1, %28.4, %18.9, %17.9, %9.4, %9.4 ve %3.2 olarak bulunmuştur. E test sonuçları ile disk difüzyon bulguları birbiri ile uyumlu bulunmuştur. S. aureus izolatlarının çoklu ilaç direnci %29.5 bulunmuştur. Vankomisin dışında izolatların antibiyotiklere dirençlilikleri hususunda PCR ile konvansiyonel metotlar arasında uyum tespit edilmiştir. Ayrıca test edilen tüm izolatların en az bir antibiyotik için gen bulundurduğu gözlenmiştir. Sonuç olarak, veteriner pratikte gereksiz antibiyotik kullanımını kontrol etmek için daha etkin uygulamalar izlenmelidir ve çoklu ilaç direncine sahip S. aureus izolatlarının yayılımını ve bulaşını azaltmak için uygun kontrol önlemlerinin uygulanması gerekmektedir

HHayvansal Gıdalardan İzole Edilen Staphylococcus aureus’ların Antibiyotik Dirençlilik Gen Profilleri

In this study, the investigation of the antibiotic resistance gene profiles of Staphylococcus aureus isolates from foods of animal origin was aimed. Totally, 95 S. aureus strains, obtained during a period between 2009 and 2012, from culture collection of the Food Hygiene and Technology Laboratory, were examined. The isolates were confirmed by phenotypic tests and PCR. The antibiotic susceptibilities of the isolates were analyzed by disc diffusion method and the minimal inhibition concentrations of the antibiotics were determined by E test. PCR were also utilized for determining the presence of resistance genes including blaZ, ermA, ermC, tetK, tetM, mecA, VanA, VanB, VatA, VatB and aacA-aphD. Resistance to penicillin, tetracycline, vancomycin, erythromycin, cefoxitin, gentamycin and quinupristin-dalfopristin were evident as 81.1%, 28.4%, 18.9%, 17.9%, 9.4%, 9.4% and 3.2% respectively. E test results were compatible with the disc diffusion method. Multidrug resistance was observed from 29.5% of S. aureus isolates. Positive compatibility was observed between conventional methods and PCR for the resistance of the isolates, except for vancomycin. In addition, all of the tested isolates found to include a resistance gene for at least one antibiotic. In conclusion, more efficient interventions must be followed to control the redundant use of antibiotics in veterinary practice. Furthermore, appropriate control measures are needed to be implemented to reduce contamination and the spread of multiresistant S. aureus strains

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