Age Related Histopathological and Immunohistochemical Changes in Horse Brains

Bu çalışmanın amacı at beyinlerinde yaşa bağlı oluşan patolojik bulguların araştırılmasıdır. Sunulan çalışmada 10 adet genç at; yaşları 1016 arasında, 13 adet yaşlı at; yaşları 17-21 arasında ve 7 adet genç at; yaşları 4-7 arasında (3-6 yaş arası) atlara ait beyinler makroskobik, histopatolojik ve immunohistokimyasal olarak incelendi. Genç atlara ait beyinlerle kıyaslandığında, yaşlı atlara ait beyinlerde makroskobik olarak ventriküler dilatasyon dikkat çekerken mikroskobik olarak satellitozis, nöronal vakuolasyon, status spongiozis, ventriküler genişleme ve ependimal ondülasyon, lipofuksin pigmenti, kalsiyum birikimleri ve aksonal şişmeler gözlendi. İmmunohistokimyasal boyamalarda yaşlı atların beyinlerinde Glial Fibrillary Acidic Protein (GFAP), ubiquitin, 2',3'-Cyclic Nucleotide 3'-Phosphodiesterase (CNPase), neuron-specific enolase (NSE), ?-amyloid protein (A?), tirozin hidroksilaz antikorları ile pozitif boyanmalar gözlendi

Atlarda Yaşla İlgili Beyinde Oluşan Histopatolojik ve İmmunohistokimyasal Değişiklikler

The aim of this study was to investigate age dependent pathological changes in horse brains. In the study, 10 mature horses aged between 10-16 years, 13 old horses aged between 17-21 years old and 7 young horses between 4-7 years old were examined macroscopically, histopathologically and immunohistohemically. As compared young horse brains; ventricular dilatation, satellitosis, neuronal vacuolization, status spongiosis, ventricular dilatation and ependymal undulation, calcium deposits and axonal swellings were seen in old horse brains. Immunohistochemical staining was obtained with Glial Fibrillary Acidic Protein (GFAP), 2’,3’-Cyclic Nucleotide 3’-Phosphodiesterase (CNPase), neuron-specific enolase (NSE), β-amyloid protein (Aβ) antibodies to indicate age related changes in brains

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