Cornea, the primary refractive element of the eye is a clear, transparent and elastic tissuethat acts as a physical barrier between the external environment and the internal ocularelements. Cornea consists of five layers. Its Stroma layer is rich with proteins especiallywith collagen. Collagen is among the most abundant fibrous proteins and fulfils a variety ofmechanical functions. When cross-links between the collagen fibers are weak, cornea willtend to thin and be deformed. This kind of eye disease is called Keratoconus.A unique treatment for Keratoconus is called corneal cross-linking (CLX). During the CLXprocedure the cornea is exposed to UV-A light and ribofilavin-dextran solution is instilledonto the cornea [1]. This treatment works by increasing collagen cross-links in the Stromalayer. CXL method has some problems which are explained in reference [2].In this study, temperature and pH level change were examined for different solutions. Ninedifferent solutions with varying concentrations were prepared by using riboflavin, dextranand dextrin substances. Behavior of these solutions at different temperatures andeffectiveness of these concentrations according to eye's pH level were investigated. Theirabsorption and fluorescence spectrum were also investigated in the critical concentration%0.1 (w/v) of riboflavin [3]. Their diffusion coefficients were found from the ratio ofluminescence intensities measurement by using Fick’s Diffusion Law.

Cornea, the primary refractive element of the eye is a clear, transparent and elastic tissuethat acts as a physical barrier between the external environment and the internal ocularelements. Cornea consists of five layers. Its Stroma layer is rich with proteins especiallywith collagen. Collagen is among the most abundant fibrous proteins and fulfils a variety ofmechanical functions. When cross-links between the collagen fibers are weak, cornea willtend to thin and be deformed. This kind of eye disease is called Keratoconus.A unique treatment for Keratoconus is called corneal cross-linking (CLX). During the CLXprocedure the cornea is exposed to UV-A light and ribofilavin-dextran solution is instilledonto the cornea [1]. This treatment works by increasing collagen cross-links in the Stromalayer. CXL method has some problems which are explained in reference [2].In this study, temperature and pH level change were examined for different solutions. Ninedifferent solutions with varying concentrations were prepared by using riboflavin, dextranand dextrin substances. Behavior of these solutions at different temperatures andeffectiveness of these concentrations according to eye's pH level were investigated. Theirabsorption and fluorescence spectrum were also investigated in the critical concentration%0.1 (w/v) of riboflavin [3]. Their diffusion coefficients were found from the ratio ofluminescence intensities measurement by using Fick’s Diffusion Law.