ENDEMİK, NESLİ TEHLİKE ALTINDA OLAN AUBRIETA OLYMPICA BOISS. BİTKİ TÜRÜNÜN IN VITRO ORTAMDA ÇOĞALTILMASI
Türkiye
coğrafik özelliklerinden dolayı zengin bir biyolojik çeşitliliğe sahiptir.
Çalışma alanımız olan Uludağ, Türkiye’nin Önemli Bitki Alanlarından birisidir. Bununla
birlikte, doğal bitki türlerimizin nesilleri, çeşitli çevresel ve insan
faktörlerinden dolayı tehlike altındadır. Çalışmamızda, Uludağ endemiği olan ve
nesli tehlike altında olan Aubrieta
olympica türünün doku kültürü şartlarında çoğaltılma imkanı
araştırılmıştır. Farklı agar konsantrasyonları ve farklı pH değerlerinin sürgün
gelişimi üzerine etkileri değerlendirilmiştir. Çoğaltma aşaması süresince en
iyi sürgün gelişimi % 0,6 agar ve pH 5,7-5,8’de meydana gelmiştir. A.
olympica bitkisinde sürgün rejenerasyonu için en iyi eksplant
kaynağının sürgün ucu ve internod olduğu tespit edilmiştir. En iyi sürgün
rejenerasyonu 0,5 ppm Kinetin ve büyüme düzenleyici içermeyen içeren Murashige
and Skoog besin ortamlarında elde edilmiştir. Yeterli uzunluğa ulaşmış
sürgünler farklı konsantrasyonlarda indol-3-bütirik asit, indole-3-asetik asit
ve büyüme düzenleyici içermeyen besin ortamında köklendirilmiştir. Bunun
yanında, A.olympica sürgünleri
bitki büyüme düzenleyici içermeyen besin ortamında kendiliğinden
köklenmiştir. Köklenen sürgünlerin sera şartlarına başarılı bir şekilde
adaptasyonu sağlanmıştır. Bu çalışma sonuçları, diğer endemik ve nesli
tükenmekte olan bitkilerde yapılacak olan koruma çalışmalarına temel
oluşturacaktır.
Anahtar Kelimeler:
Çoğaltma, Uludağ, Bitki büyüme düzenleyiciler
IN VITRO PROPAGATION OF ENDEMIC, ENDANGERED PLANT SPECIES AUBRIETA OLYMPICA BOISS.
Turkey
has a rich biological diversity due to its geographical feature. Uludağ
mountain is one of the Important Plant Areas of Turkey. Thus, native plant
species are threatened due to various environmental factors and human damage.
The micropropagation possibility for Aubrieta
olympica Uludağ endemic threatened plant species, were investigated in
this study. The effect of different concentrations of agar and different levels
of pH were evaluated on shoot development. During the proliferation stage, the
best development occured at 0.6% agar and pH 5.7-5.8. Shoot tips and internode
explants were the best source for the highest shoot induction of A.olympica species. The best results for
shoot formation were obtained with 0.5 ppm Kinetin and plant growth
regulators free Murashige and Skoog medium. Elongated shoots were
rooted in the Murashige and Skoog medium supplemented with different concentrations
of indole-3-butyric acid, indole-3-acetic acid, and also a medium without plant
growth regulators. However, A.olympica shoots spontaneously rooted on plant growth
regulators free medium. Shoots which developed roots were transferred to
greenhouse, successfully. The results of this study could be utilized for
conservation purposes of endemic and endangered plant species.
Keywords:
Micropropagation, Uludağ, Plant growth regulators,
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- [1] Güleryüz, G., Malyer, H., Kaynak, G. and Özhatay, N., “Uludağ A2 (A) Bursa. In: Important Plant Areas in Turkey: 122 Key Turkish Botanical Sites”, Editors Byfield, A., Atay, S., Özhatay, N., WWF Turkey, İstanbul, pp. 77-79, (2010).
- [2] Urbanska, K.M. and Fattorini, M., “Seed rain in high-altitude restoration plots in Switzerland”, Restoration Ecology, 8:74-79, (2000).
- [3] Pintar, M., Mali, B. and Kraigher H., “The impact of ski slopes management on Krvavec Ski Resort (Slovenia) on hydrological function of soils”, Biologia, 64:639-642, (2009).
- [4] Güleryüz, G., Kırmızı, S., Arslan, H. and Kondu, Yakut E., “Alterations of the nitrojen mineralization rates in soils of forest community depending on the ski run constructions (Mount Uludağ, Bursa, Turkey)”, Journal of Mountain Science, 8:53-61, (2011).
- [5] Güleryüz, G., “Aubrieta olympica Boiss. Brassicaceae/Cruciferae (Lahanagiller)”, The Karaca Arboretum Magazine, 5:45-48, (1999).
- [6] Ekim, T., Koyuncu, M., Vural, M., Duman, H., Aytaç, Z. and Adıgüzel, N., “Red Data Book of Turkish Plants (Pteridophyta and Spermatophyta)” Turkish Association for the Conservation of Nature, Ankara, (2000).
- [7] Güleryüz, G, “Alpine Flower’s of Uludağ, Bursa. Governorship of Bursa Provincial Directorate of Tourism”, Bursa, pp.168, (2000).
- [8] Rao, N.K., “Plant genetic resources: Advancing conservation and use through biotechnology”, African Journal of Biotechnology, 3:136-145, (2004).
- [9] Kleps, C., “Sustainable agriculture – The sole alternative for the present environmental and climatic constrains”, Journal of Environmental Protection Ecology, 10:1187-1193, (2009).
- [10] Erisen, S., Yorgancılar, M., Atalay, E., Babaoglu, M. and Duran, A., “Callus induction and plant regeneration of the endemic Astragalus nezaketae in Turkey”, Electronic Journal of Biotechnology, 13:1-7, (2010).
- [11] Kasso, M. and Balakrishnan, M.. “Ex situ conservation of biodiversity with particular emphasis to Ethiopia”, Hindawi Pub.Corporation, Article ID 985037:1-11, (2013).
- [12] Akın, B. and Kocaçalışkan, İ., “In vitro propagation of Arabis drabiformis Boiss. (Brassiaceae) an endemic rare species of Uludağ Mountain (Bursa-Turkey)”, African Journal of Biotechnology, 10:18356-18361, (2011).
- [13] Akın, B., Kocaçalışkan, İ. and Güleryüz, G., “Micropropagation of Erodium sibthorpianum subsp. sibthorpianum an endemic threatened species of Uludağ mountain (Bursa-Turkey)”, Turkish Journal of Botany, 38:148-155, (2014).
- [14] Sudha, C.G. and Seeni, S. “In Vitro propagation of Rauvolfia micrantha, a rare medicinal plant”, Plant Cell, Tissue and Organ Culture, 44:243-248, (1996).
- [15] Sudha, C.G., Krishnan, P.N. and Pushpangadan, P.,“In Vitro propagation of Holostemma annulare (Roxb.) K. Schum., a rare medicinal plant”, In Vitro Cellular & Developmental Biology - Plant., 34:57-63, (1998).
- [16] Fay, M.F., “Conservation of rare and endangered plants using In Vitro methods”, In Vitro Cellular & Developmental Biology - Plant., 28:1-4, (1992).
- [17] Murashige, T. and Skoog, F., “A revised medium for rapid growth and bioassays with tobacco tissue cultures”, Physiologia Plantarum, 15:473-497, (1962).
- [18] Kocaçalışkan, İ., “Plant Tissue Culture (In Turkish)”, Bizim Büro Press, Ankara-Turkey, (2008).
- [19] Razdan, M.K., “Introduction to Plant Tissue Culture Second Edition. Science Publishers”, United States of America, (2003).
- [20] Morgan, E.R., Butler, R.M. and Bicknell RA, “In Vitro propagation of Gentiana cerina and Gentiana corymbifera”, New Zealand Journal of Crop and Horticultural Science, 25:1-8, (1997).
- [21] Karim, M.Z., Yokota, S., Rahman, M.M., Eizawa, J., Saito, Y., Azad, M.A.K., Ishiguri, F., Iizuka, K. and Yoshizawa, N., “Effects of sucrose concentration and pH level on shoot regeneration from callus in Araria elata Seem”, Asian Journal of Plant Sciences, 6:715-717, (2007).
- [22] George, E.F., “The Components of Culture Media. In: Plant Propagation by Tissue Culture”, Editor George, E., Exegetics Ltd., England, pp. 274-338, (1993).
- [23] Ebrahim, M.K.H. and Ibrahim, I.A., “Influence of medium solidification and pH value on In Vitro propagation of Maranta leuconeura cv. Kerchoviana”, Scientia Horticulturae (Amsterdam, Neth.), 86:211-221, (2000).
- [24] Gurel, S. and Gulsen, Y., “The effects of different sucrose, agar and pH levels on in vitro shoot production of Almond (Amygdalus communis L.)”, Turkish Journal of Botany, 22: 363-373, (1998).
- [25] Finn, C.E., Luby, J.J., Rosen, C.J. and Ascher, P.D., “Evaluation in vitro of blueberry germplasm for higher pH tolerance”, Journal of the American Society for Horticultural Science, 116:312-316, (1991).
- [26] Suthar, R.K., Habibi, N. and Purohit, S.D., “Influence of agar concentration and liquid medium on in vitro propagation of Boswellia serrata Roxb.”, Indian Journal of Biotechnology, 10:224-227, (2011).
- [27] Abdoli, M., Moieni, A. and Dehghani, H., “Effects of cultivar and agar concentration on in vitro shoot organogenesis and hyperhydricity in sunflower (Helianthus annuus L.)”, Pakistan Journal of Botany, 39:31-35, (2007).
- [28] Saad, A.I.M. and Elshahed, A.M., “Plant Tissue Culture Media. In: Recent Advances in Plant In Vitro Culture”, Editors Leva, A., Rinaldi, L.M.R., InTech Publisher, pp. 219, (2012).
- [29] Rout, G.R., “Effect of cytokinins and auxins on micropropagation of Clitoria ternatea L. Biology Letters”, 41:21-26, (2004).
- [30] Bhojwani, S.S., Razdan, M.K., “Clonal Propagation, Plant Tissue Culture: Theory and Practise, a Revised Edition”, Elsevier, Amsterdam, (1996).
- [31] Babaoğlu, M., Gürel, E. and Özcan, S., “Plant Biotechnology I, Tissue Culture and its Applications”, S. U. Press, Konya, (2001).
- [32] Sarasan, V., Cripps, R., Ramsay, M.M., Atherton, C., Mcmichen, M., Prendergast, G. and Rowntree, J.K., “Conservation in vitro of threatened plants-progress in the past decade”, In Vitro Cellular & Developmental Biology - Plant., 42:206-214, (2006).
- [33] Mikulík, J., “Propagation of endangered plant species by tissue cultures”, Acta Universitatis Palackianae Olomucensis Facultas Rerum Naturalium Biologica, 37:27-33, (1999).
- [34] Tefera, W. and Wannakrairoj, S., “Synergistic effects of some plant growth regulators on in vitro shoot proliferation of Korarima (Aframomum corrorima (Braun) Jansen)”, African Journal of Biotechnology, 5:1894-1901, (2006).
- [35] Pérez-bermúdez, P., Seitz, H. and Gavidia, I., “A protocol for rapid micropropagation of endangered Isoplexis”, In Vitro Cellular & Developmental Biology - Plant.,38:178-182, (2002).
- [36] Kļaviņa, D., Gailîte, A., Jakobsone, G., Necajeva, J. and Gavrilova, Ì., “Tissue culture technology in conservation of threatened plant species of Latvia”, Acta Universitatis Latviensis ser. Biology, 676:183-188, (2004).
- [37] Sivanesan, I., Hwang, S.J.and Jeong, B.R., “Influence of plant growth regulators on axillary shoot multiplication and iron source on growth of Scrophularia takesimensis Nakai - a rare endemic medicinal plant”, African Journal of Biotechnology, 7:4484-4490, (2008).
- Başlangıç: 2020
- Yayıncı: Kütahya Dumlupınar Üniversitesi