SURVEY OF GENE EXPRESSION DURING UREDOSPORE GERMINATION IN PUCCINIA SORGHI

Transcript profiling is commonly used to identify genes that are expressed under various conditions, in different tissues and developmental stages of organisms. Transcripts tags detected from germinating uredospores of Puccinia sorghi T09 isolate using a modified cDNA-AFLP approach were characterized in the current study. GenBank similarity searches and sequence mapping of 56 sequence tags to available genome sequence of Argentinian strain RO10H11247 revealed 38 P. sorghi similar sequences, corresponding to 31 individual Genbank records. With the obtained similarities and protein domain searches, presumed functions associated with the 27 Ps TDFs were inferred, the majority of which appear to encode products that appear to be important in host invasion, pathogen growth and proliferation. Among them, chitinase, oligopeptide transporter protein, peptidyl-tRNA hydrolase, signal peptide, small secreted proteins, velvet and cutinase domain containing proteins are the prominent ones. Expressions of four selected genes, three of which are newly identified, were verified in germinating spores and infected plant leaf material cDNA preparations with RT-sqPCR. Together with the newly identified and annotated P. sorghi genes, obtained profile, in general, represent a gene set whose products are conceivably involved in host invasion and pathogenesis along with the basic housekeeping functions.

Keywords:

P. sorghi, germinating uredospore, , cDNA-AFLP Expressed Sequence Tag,

PDF

___

[1] Sahah, D. A., Dillard, H. (2006), Yield loss in sweet corn caused by Puccinia sorghi: A metaanalysis. Plant Disease, 90, 1413-1418.
[2] Hooker, A. L. (1969), Widely based resistance to rust in corn. In: Browning J. A., editor. Disease Consequences of Intensive and Extensive Culture of Field Crops. Special Report 64, Agricultural and Home Economics Experiment Station. Ames, IA, USA: Iowa State University of Science and Technology, pp. 28–34.
[3] Hulbert, S. H. (1997), Structure and evolution of the rp1 complex conferring rust resistance in maize. Annual Review of Phytopathology, 35, 293–310.
[4] Hahn, M., Mendgen, K. (1997), Characterization of in planta-induced rust genes isolated from a haustorium-specific cDNA library. Molecular Plant Microbe Interactions, 10, 427-437.
[5] Hu, G. G., Linning, R., Mccallum, B., Banks, T., Cloutier, S., and Bakkeren, G. (2007), Generation of a wheat leaf rust, Puccinia triticina, EST database from stage-specific cDNA libraries. Molecular Plant Pathology, 8, 451-467.
[6] Zhang, Y., Qu, Z., Zheng, W., Liu, B., Wang, X., and Kang, Z. (2008), Stage-specific gene expression during urediniospore germination in Puccinia striiformis f. sp tritici. BMC Genomics, 9, 203- doi:10.1186/1471-2164-9-203.
[7] Soanes, D. M., Talbot, N. J. (2006), Comparative genomic analysis of phytopathogenic fungi using expressed sequence tag (EST) collections. Molecular Plant Pathology, 7(1), 61-70.
[8] Lakshman, D. K., Alkharouf, N., Roberts, D. P., Natarajan, S. S., Mitra, A. (2012), Gene expression profiling of the plant pathogenic basidiomycetous fungus Rhizoctonia solani AG 4 reveals putative virulence factors. Mycologia, 104 (5), 1020-1035.
[9] Stergiopoulos, I., de Wit, P. J. G. M. (2009), Fungal Effector Proteins. Annual Review of Phytopathology, 47, 233–63.
[10] Rouxel, T., Baledent, M. H. (2010), Avirulance genes. In Encyclopedia of Life Sciences (eLS), John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1002/9780470015902.a0021267].
[11] Tang, C., Xu, Q., Zhao, M., Wang, X., Kang, Z. (2018), Understanding the lifestyles and pathogenicity mechanisms of obligate biotrophic fungi in wheat: The emerging genomics era. The Crop Journal, 6, 60-67.
[12] Rochi, L., Diéguez, M. J., Burguener, G., Darino, M. A., Pergolesi, M. F., and Sacco, F. (2018), Characterization and comparative analysis of the genome of Puccinia sorghi Schwein, the causal agent of maize common rust. Fungal Genetics and Biology, 112, 31–39.
[13] Howe, K. L., Contreras-Moreira, B., De Silva, N., Maslen, G., Akanni, W., and Cambell, L. (2019), Ensembl Genomes 2020-enabling non-vertebrate genomic research. Nucleic Acids Research 48, database issue, D689–D695, doi: 10.1093/nar/gkz890.
[14] Südüpak, M. A. (2014), A cDNA-AFLP protocol with reciprocally arranged 2-enzyme sequential digestion and silver staining detection. Turkish Journal of Biology, 38, 260-270.
[15] Petersen, T. N., Brunak, S., von Heijne, G., Nielsen, H. (2011), SignalP 4.0: discriminating signal peptides from transmembrane regions. Nature Methods, 8, 785–786.
[16] Lin, F., Jiang, L., Liu, Y., Lv, Y., Dai, H., Zhao, H. (2014), Genome‑wide identification of housekeeping genes in maize. Plant Molecular Biology, 68, 543-554.
[17] Upadhyaya, N. M., Garnica, D. P., Karaoglu, H., Sperschneider, J., Nemri, A., Xu, B., and Dodds, P. N. (2014), Comparative genomics of Australian isolates of the wheat stem rust pathogen Puccinia graminis f. sp. tritici reveals extensive polymorphism in candidate effector genes. Frontiers in Plant Sciences, 5, 759-.
[18] Cuomo, C. A., Bakkeren, G., Khalil, H. B., Panwar, V., Joly, D., and Fellers, J. P. (2017), Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci. G3-Genes Genomes Genetics, 7, 361–376.
[19] Duplessis, S., Cuomo, C. A., Lin, Y. C., Aerts, A., Tisserant, E., and Martin, F. (2011), Obligate biotrophy features unraveled by the genomic analysis of rust fungi. Proc. Natl. Acad. Sci. U. S. A. 108, 9166–9171.
[20] López-Berges, M. S., Concepción, H., Michael, S., Katja, S., Javier, C., and Antonio, D. (2012), The velvet complex governs mycotoxin production and virulence of Fusarium oxysporum on plant and mammalian hosts. Molecular Microbiology, 87 (1), 49–65.
[21] Wang, R., Leng, Y., Shrestha, S., Zhong, S. (2016), Coordinated and independent functions of velvet-complex genes in fungal development and virulence of the fungal cereal pathogen Cochliobolus sativus. Fungal Biology, 120 (8),948–960.
[22] Idnurm, A., Howlett, B. J. (2001), Pathogenicity genes of phytopathogenic fungi. Molecular Plant Pathology, 2, 241-255.
[23] Zhang, Y., Qu, Z., Zheng, W., Liu, B., Wang, X., and Kang, Z. (2008), Stage-specific gene expression during urediniospore germination in Puccinia striiformis f. sp tritici. BMC Genomics, 9, 203- doi:10.1186/1471-2164-9-203
[24] Sharma, S., Kaushik, S., Sinha, M., Kushwaha, G. S., Singh, A., and Singh, P. (2014), Structural and functional insights into peptidyl-tRNA hydrolase. Biochimica et Biophysica Acta, 1844, 1279–1288.
[25] Voegele, R. T., Hahn, M., Mendgen, K. (2009), The uredinales: cytology, biochemistry, and molecular biology. The Mycota, 5. Plant relationships/Vol. ed. H. B. Deising. Berlin: Springer, 2. ed., pp. 69-98.

Başlangıç: 2020
Yayıncı: Kütahya Dumlupınar Üniversitesi

Arşiv